4HLN

Structure of barley starch synthase I in complex with maltooligosaccharide

PDB DOI: https://doi.org/10.2210/pdb4HLN/pdb

Classification: TRANSFERASE
Organism(s): Hordeum vulgare
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2012-10-17 Released: 2013-06-05
Deposition Author(s): Cuesta-Seijo, J.A., Nielsen, M.M., Marri, L., Tanaka, H., Palcic, M.M.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.70 Å
R-Value Free: 0.199
R-Value Work: 0.173
R-Value Observed: 0.174

wwPDB Validation 3D Report Full Report

This is version 2.0 of the entry. See complete history.

Macromolecule Content

Total Structure Weight: 70.73 kDa
Atom Count: 4,058
Modelled Residue Count: 504
Deposited Residue Count: 633
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Starch synthase I	A	633	Hordeum vulgare	Mutation(s): 0 Gene Names: SSI, glgA2 EC: 2.4.1.21
UniProt
Find proteins for Q9M5A3 (Hordeum vulgare) Explore Q9M5A3 Go to UniProtKB: Q9M5A3
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q9M5A3
Sequence Annotations Expand
Reference Sequence

Oligosaccharides

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Entity ID: 2
Molecule	Chains	Length	2D Diagram	Glycosylation	3D Interactions
alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	B	5		N/A	Interactions Focus chain B Interactions & Density Focus chain B
Glycosylation Resources
GlyTouCan: G50146AM GlyCosmos: G50146AM

Biologically Interesting Molecules (External Reference) 1 Unique

Entity ID: 2
ID	Chains	Name	Type/Class	2D Diagram	3D Interactions
PRD_900030 Query on PRD_900030	B	alpha-maltopentaose	Oligosaccharide / Substrate analog		Interactions Focus chain B Interactions & Density Focus chain B

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.70 Å
R-Value Free: 0.199
R-Value Work: 0.173
R-Value Observed: 0.174
Space Group: P 3₁ 2 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 153.15	α = 90
b = 153.15	β = 90
c = 84.96	γ = 120

Software Package:

Software Name	Purpose
ADSC	data collection
PHASER	phasing
PHENIX	refinement
XDS	data reduction
XDS	data scaling

Structure Validation

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Entry History

Deposition Data

Released Date: 2013-06-05

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2013-06-05
Type: Initial release
Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Advisory, Atomic model, Data collection, Database references, Derived calculations, Structure summary

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Help and Resources

4HLN

Structure of barley starch synthase I in complex with maltooligosaccharide

Entity ID: 1

UniProt

Entity Groups

Sequence Annotations

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Entity ID: 2

Glycosylation Resources

Entity ID: 2

Experimental Data

Structure Validation

Deposition Data

Revision History (Full details and data files)