4FP7

2.2A resolution structure of Proteasome Assembly Chaperone Hsm3

PDB DOI: https://doi.org/10.2210/pdb4FP7/pdb

Classification: CHAPERONE
Organism(s): Saccharomyces cerevisiae S288C
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2012-06-21 Released: 2013-04-10
Deposition Author(s): Lovell, S., Battaile, K.P., Singh, R., Zolkiewski, M., Roelofs, J.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.20 Å
R-Value Free: 0.239
R-Value Work: 0.188
R-Value Observed: 0.191

wwPDB Validation 3D Report Full Report

This is version 1.2 of the entry. See complete history.

Literature

2.2A resolution structure of Proteasome Assembly Chaperone Hsm3

Singh, R., Lovell, S., Zolkiewski, M., Battaile, K.P., Roelofs, J.

To be published.

Macromolecule Content

Total Structure Weight: 113.37 kDa
Atom Count: 7,286
Modelled Residue Count: 870
Deposited Residue Count: 982
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
DNA mismatch repair protein HSM3	A, B	491	Saccharomyces cerevisiae S288C	Mutation(s): 0 Gene Names: HSM3, YBR272C, YBR1740
UniProt
Find proteins for P38348 (Saccharomyces cerevisiae (strain ATCC 204508 / S288c)) Explore P38348 Go to UniProtKB: P38348
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P38348
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.20 Å
R-Value Free: 0.239
R-Value Work: 0.188
R-Value Observed: 0.191
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 85.148	α = 90
b = 94.466	β = 90
c = 129.931	γ = 90

Software Package:

Software Name	Purpose
SCALA	data scaling
PHASER	phasing
PHENIX	refinement
PDB_EXTRACT	data extraction
JDirector	data collection
XDS	data reduction
PHENIX	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2013-04-10

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2013-04-10
Type: Initial release
Version 1.1: 2017-11-15
Changes: Refinement description
Version 1.2: 2024-02-28
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.