4FKE
Crystal structure of porcine aminopeptidase-N
- PDB DOI: https://doi.org/10.2210/pdb4FKE/pdb
- Classification: HYDROLASE
- Organism(s): Sus scrofa
- Expression System: Spodoptera frugiperda
- Mutation(s): No
- Deposited: 2012-06-13 Released: 2012-10-17
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.182
- R-Value Work: 0.142
- R-Value Observed: 0.144
This is version 2.0 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Aminopeptidase N | 909 | Sus scrofa | Mutation(s): 0 Gene Names: ANPEP EC: 3.4.11.2 |  | |
UniProt | |||||
Find proteins for P15145 (Sus scrofa) Explore P15145 Go to UniProtKB: P15145 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P15145 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Oligosaccharides
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | 2D Diagram | Glycosylation | 3D Interactions |
| 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | B, C, F | 3 |  | N-Glycosylation | |
Glycosylation Resources | |||||
GlyTouCan: G47362BJ GlyCosmos: G47362BJ GlyGen: G47362BJ | |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| NAG Query on NAG | K [auth A], L [auth A] | 2-acetamido-2-deoxy-beta-D-glucopyranose C8 H15 N O6 OVRNDRQMDRJTHS-FMDGEEDCSA-N |  | ||
| ZN Query on ZN | M [auth A] | ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N |  | ||
Biologically Interesting Molecules (External Reference) 1 Unique
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| ID | Chains | Name | Type/Class | 2D Diagram | 3D Interactions |
| PRD_900017 Query on PRD_900017 | B, C, F | triacetyl-beta-chitotriose | Oligosaccharide / Inhibitor |  | |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.182
- R-Value Work: 0.142
- R-Value Observed: 0.144
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 259.971 | α = 90 |
| b = 62.77 | β = 100.12 |
| c = 81.794 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| SOLVE | phasing |
| CNS | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
| HKL-2000 | data collection |
Entry History
Deposition Data
Revision History (Full details and data files)
- Version 1.0: 2012-10-17
Type: Initial release - Version 1.1: 2012-10-31
Changes: Database references, Structure summary - Version 1.2: 2012-11-14
Changes: Database references - Version 1.3: 2019-07-17
Changes: Data collection, Derived calculations, Refinement description - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Advisory, Atomic model, Data collection, Database references, Derived calculations, Structure summary
