4DN6

Crystal structure of a putative pilus assembly protein (cpaE) from Burkholderia thailandensis E264 at 2.80 A resolution

PDB DOI: https://doi.org/10.2210/pdb4DN6/pdb

Classification: SIGNALING PROTEIN
Organism(s): Burkholderia thailandensis E264
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2012-02-08 Released: 2012-02-29
Deposition Author(s): Joint Center for Structural Genomics (JCSG), Shapiro, L.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.80 Å
R-Value Free: 0.239
R-Value Work: 0.213
R-Value Observed: 0.214

wwPDB Validation 3D Report Full Report

This is version 1.4 of the entry. See complete history.

Literature

Crystal structure of a putative pilus assembly protein (cpaE) from Burkholderia thailandensis E264 at 2.80 A resolution

Joint Center for Structural Genomics (JCSG), Shapiro, L.

To be published.

Macromolecule Content

Total Structure Weight: 32.64 kDa
Atom Count: 1,864
Modelled Residue Count: 248
Deposited Residue Count: 292
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Putative pilus assembly protein CpaE	A, B	146	Burkholderia thailandensis E264	Mutation(s): 0 Gene Names: cpaE, BTH_II2273
UniProt
Find proteins for Q2T2Y8 (Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CCUG 48851 / CIP 106301 / E264)) Explore Q2T2Y8 Go to UniProtKB: Q2T2Y8
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q2T2Y8
Sequence Annotations Expand
Reference Sequence

Small Molecules

Modified Residues 1 Unique
ID	Chains	Type	Formula	2D Diagram	Parent
MSE Query on MSE	A, B	L-PEPTIDE LINKING	C₅ H₁₁ N O₂ Se		MET

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.80 Å
R-Value Free: 0.239
R-Value Work: 0.213
R-Value Observed: 0.214
Space Group: P 4₂ 2₁ 2
Diffraction Data: https://doi.org/10.18430/M34DN6

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 116.114	α = 90
b = 116.114	β = 90
c = 45.83	γ = 90

Software Package:

Software Name	Purpose
MolProbity	model building
PDB_EXTRACT	data extraction
SHELX	phasing
SHARP	phasing
XSCALE	data scaling
BUSTER-TNT	refinement
XDS	data reduction
SHELXD	phasing
BUSTER	refinement

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2012-02-29

Deposition Author(s):

Joint Center for Structural Genomics (JCSG)

Revision History (Full details and data files)

Version 1.0: 2012-02-29
Type: Initial release
Version 1.1: 2012-03-14
Changes: Other
Version 1.2: 2017-11-15
Changes: Refinement description
Version 1.3: 2018-01-24
Changes: Database references
Version 1.4: 2023-02-01
Changes: Database references, Derived calculations

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.