4Z8W

Crystal Structure of the Major Plantain Pollen Allergen Pla l 1

PDB DOI: https://doi.org/10.2210/pdb4Z8W/pdb

Classification: ALLERGEN
Organism(s): Plantago lanceolata
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2015-04-09 Released: 2016-07-06
Deposition Author(s): Freier, R., Brandstetter, H.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.98 Å
R-Value Free: 0.248
R-Value Work: 0.217
R-Value Observed: 0.218

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Crystal structure of Pla l 1 reveals both structural similarity and allergenic divergence within the Ole e 1-like protein family.

Stemeseder, T., Freier, R., Wildner, S., Fuchs, J.E., Briza, P., Lang, R., Batanero, E., Lidholm, J., Liedl, K.R., Campo, P., Hawranek, T., Villalba, M., Brandstetter, H., Ferreira, F., Gadermaier, G.

(2017) J Allergy Clin Immunol 140: 277-280

PubMed: 27965108 Search on PubMedSearch on PubMed Central
DOI: https://doi.org/10.1016/j.jaci.2016.10.035
Primary Citation of Related Structures:
4Z8W

Organizational Affiliation

Department of Molecular Biology, University of Salzburg, Salzburg, Austria.

Macromolecule Content

Total Structure Weight: 14.61 kDa
Atom Count: 1,056
Modelled Residue Count: 125
Deposited Residue Count: 131
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Major pollen allergen Pla l 1	A	131	Plantago lanceolata	Mutation(s): 0
UniProt
Find proteins for P82242 (Plantago lanceolata) Explore P82242 Go to UniProtKB: P82242
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P82242
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
ZN Query on ZN Download Ideal Coordinates CCD File SDF format, chain B [auth A] MOL2 format, chain B [auth A]	B [auth A]	ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N		Interactions Focus chain B [auth A] Interactions & Density Focus chain B [auth A]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.98 Å
R-Value Free: 0.248
R-Value Work: 0.217
R-Value Observed: 0.218
Space Group: P 4₃ 2₁ 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 49.64	α = 90
b = 49.64	β = 90
c = 130.09	γ = 90

Software Package:

Software Name	Purpose
SCALA	data scaling
SHELX	phasing
REFMAC	refinement
PDB_EXTRACT	data extraction
MOSFLM	data reduction

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2016-07-06

Deposition Author(s):

Brandstetter, H.

Revision History (Full details and data files)

Version 1.0: 2016-07-06
Type: Initial release
Version 1.1: 2018-05-30
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.