Download MendeleyCrystal structure of sugar aminotransferase WecE from Escherichia coli K-12
Wang, F., Xu, W., Helmich, K.E., Singh, S., Yennamalli, R.M., Bingman, C.A., Miller, M.D., Thorson, J.S., Phillips Jr., G.N.To be published.
4PIW
Crystal structure of sugar aminotransferase WecE from Escherichia coli K-12
- PDB DOI: https://doi.org/10.2210/pdb4PIW/pdb
- Classification: TRANSFERASE
- Organism(s): Escherichia coli K-12
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2014-05-09 Released: 2014-07-16
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.70 Å
- R-Value Free: 0.275
- R-Value Work: 0.236
- R-Value Observed: 0.237
wwPDB Validation 3D Report Full Report
This is version 2.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| TDP-4-keto-6-deoxy-D-glucose transaminase family protein | 396 | Escherichia coli K-12 | Mutation(s): 0 Gene Names: wecE |  | |
UniProt | |||||
Find proteins for P27833 (Escherichia coli (strain K12)) Explore P27833 Go to UniProtKB: P27833 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P27833 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Modified Residues 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| LLP Query on LLP | A, B, C, D, E A, B, C, D, E, F, G, H | L-PEPTIDE LINKING | C14 H22 N3 O7 P |  | LYS |
| MSE Query on MSE | A, B, C, D, E A, B, C, D, E, F, G, H | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.70 Å
- R-Value Free: 0.275
- R-Value Work: 0.236
- R-Value Observed: 0.237
- Space Group: P 1 2 1
- Diffraction Data: https://doi.org/10.18430/m34piw
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 125.341 | α = 90 |
| b = 87.483 | β = 91.1 |
| c = 161.554 | γ = 90 |
| Software Name | Purpose |
|---|---|
| XDS | data reduction |
| XDS | data scaling |
| PHENIX | phasing |
| PDB_EXTRACT | data extraction |
| PHENIX | refinement |
| XSCALE | data scaling |
Entry History & Funding Information
Deposition Data
- Released Date: 2014-07-16 Deposition Author(s): Wang, F., Xu, W., Helmich, K.E., Singh, S., Yennamalli, R.M., Miller, M.D., Bingman, C.A., Thorson, J.S., Phillips Jr., G.N., Enzyme Discovery for Natural Product Biosynthesis (NatPro)
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | U01GM098248 |
Revision History (Full details and data files)
- Version 1.0: 2014-07-16
Type: Initial release - Version 1.1: 2015-10-14
Changes: Data collection - Version 2.0: 2017-09-27
Changes: Author supporting evidence, Derived calculations, Non-polymer description, Refinement description, Structure summary - Version 2.1: 2019-12-25
Changes: Author supporting evidence - Version 2.2: 2023-09-27
Changes: Data collection, Database references, Refinement description - Version 2.3: 2023-11-15
Changes: Data collection
