4LSA
Crystal structure of BRI1 sud1 (Gly643Glu) bound to brassinolide
- PDB DOI: https://doi.org/10.2210/pdb4LSA/pdb
- Classification: STEROID BINDING PROTEIN
- Organism(s): Arabidopsis thaliana
- Expression System: Trichoplusia ni
- Mutation(s): Yes
- Deposited: 2013-07-22 Released: 2013-09-04
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.50 Å
- R-Value Free: 0.251
- R-Value Work: 0.205
- R-Value Observed: 0.207
This is version 2.1 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Protein BRASSINOSTEROID INSENSITIVE 1 | 774 | Arabidopsis thaliana | Mutation(s): 1 Gene Names: At4g39400, BRI1, F23K16.30 |  | |
UniProt | |||||
Find proteins for O22476 (Arabidopsis thaliana) Explore O22476 Go to UniProtKB: O22476 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | O22476 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Oligosaccharides
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | 2D Diagram | Glycosylation | 3D Interactions |
| alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | B, C, D | 4 |  | N-Glycosylation | |
Glycosylation Resources | |||||
GlyTouCan: G81315DD GlyCosmos: G81315DD GlyGen: G81315DD | |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| BLD Query on BLD | I [auth A] | Brassinolide C28 H48 O6 IXVMHGVQKLDRKH-KNBKMWSGSA-N |  | ||
| NAG Query on NAG | E [auth A], F [auth A], G [auth A], H [auth A] | 2-acetamido-2-deoxy-beta-D-glucopyranose C8 H15 N O6 OVRNDRQMDRJTHS-FMDGEEDCSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.50 Å
- R-Value Free: 0.251
- R-Value Work: 0.205
- R-Value Observed: 0.207
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 174.033 | α = 90 |
| b = 67.303 | β = 121.09 |
| c = 120.901 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHASER | phasing |
| PHENIX | refinement |
| XDS | data reduction |
| XSCALE | data scaling |
Entry History
Deposition Data
- Released Date: 2013-09-04 Deposition Author(s): Santiago, J., Henzler, C., Hothorn, M.
Revision History (Full details and data files)
- Version 1.0: 2013-09-04
Type: Initial release - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Database references, Derived calculations, Structure summary - Version 2.1: 2023-09-20
Changes: Data collection, Database references, Refinement description, Structure summary
