Download MendeleyCrystal structure of a GNAT superfamily phosphinothricin acetyltransferase (Pat) from Sinorhizobium meliloti 1021
Majorek, K.A., Cooper, D.R., Ahmed, M., Stead, M., Hillerich, B., Seidel, R., Bonanno, J.B., Almo, S.C., Minor, W.To be published.
4JXQ
Crystal structure of a GNAT superfamily phosphinothricin acetyltransferase (Pat) from Sinorhizobium meliloti 1021
- PDB DOI: https://doi.org/10.2210/pdb4JXQ/pdb
- Classification: TRANSFERASE
- Organism(s): Sinorhizobium meliloti 1021
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2013-03-28 Released: 2013-05-08
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.15 Å
- R-Value Free: 0.153
- R-Value Work: 0.120
- R-Value Observed: 0.122
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Acetyltransferase | 186 | Sinorhizobium meliloti 1021 | Mutation(s): 0 Gene Names: R03223, SMc03840 EC: 2.3.1 |  | |
UniProt | |||||
Find proteins for Q92L60 (Rhizobium meliloti (strain 1021)) Explore Q92L60 Go to UniProtKB: Q92L60 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q92L60 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 4 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| 2PE Query on 2PE | E [auth A] | NONAETHYLENE GLYCOL C18 H38 O10 YZUUTMGDONTGTN-UHFFFAOYSA-N |  | ||
| FLC Query on FLC | D [auth A], H [auth B] | CITRATE ANION C6 H5 O7 KRKNYBCHXYNGOX-UHFFFAOYSA-K |  | ||
| PEG Query on PEG | F [auth B] | DI(HYDROXYETHYL)ETHER C4 H10 O3 MTHSVFCYNBDYFN-UHFFFAOYSA-N |  | ||
| EDO Query on EDO | C [auth A], G [auth B] | 1,2-ETHANEDIOL C2 H6 O2 LYCAIKOWRPUZTN-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A, B | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.15 Å
- R-Value Free: 0.153
- R-Value Work: 0.120
- R-Value Observed: 0.122
- Space Group: P 21 21 21
- Diffraction Data: https://doi.org/10.18430/M3R59W
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 51.461 | α = 90 |
| b = 80.481 | β = 90 |
| c = 83.611 | γ = 90 |
| Software Name | Purpose |
|---|---|
| HKL-3000 | data collection |
| HKL-3000 | phasing |
| SHELXD | phasing |
| SHELXE | model building |
| MLPHARE | phasing |
| DM | model building |
| ARP/wARP | model building |
| REFMAC | refinement |
| Coot | model building |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
| DM | phasing |
Entry History
Deposition Data
- Released Date: 2013-05-08 Deposition Author(s): Majorek, K.A., Cooper, D.R., Osinski, T., Ahmed, M., Stead, M., Hillerich, B., Seidel, R., Bonanno, J.B., Almo, S.C., Minor, W., New York Structural Genomics Research Consortium (NYSGRC)
Revision History (Full details and data files)
- Version 1.0: 2013-05-08
Type: Initial release - Version 1.1: 2013-05-15
Changes: Structure summary - Version 1.2: 2022-04-13
Changes: Database references, Derived calculations, Structure summary
