4IYE

Crystal structure of AdTx1 (rho-Da1a) from eastern green mamba (Dendroaspis angusticeps)

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.95 Å
  • R-Value Free: 0.252 
  • R-Value Work: 0.170 
  • R-Value Observed: 0.174 

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This is version 1.2 of the entry. See complete history. 


Literature

Crystallization of recombinant green mamba rho-Da1a toxin during a lyophilization procedure and its structure determination.

Maiga, A., Vera, L., Marchetti, C., Lorphelin, A., Bellanger, L., Mourier, G., Servent, D., Gilles, N., Stura, E.A.

(2013) Acta Crystallogr Sect F Struct Biol Cryst Commun 69: 704-709

  • DOI: https://doi.org/10.1107/S1744309113011470
  • Primary Citation of Related Structures:  
    4IYE

  • PubMed Abstract: 

    ρ-Da1a toxin from eastern green mamba (Dendroaspis angusticeps) venom is a polypeptide of 65 amino acids with a strong affinity for the G-protein-coupled α(1A)-adrenoceptor. This neurotoxin has been crystallized from resolubilized lyophilized powder, but the best crystals grew spontaneously during lyophilization. The crystals belonged to the trigonal space group P3(1)21, with unit-cell parameters a = b = 37.37, c = 66.05 Å, and diffracted to 1.95 Å resolution. The structure solved by molecular replacement showed strong similarities to green mamba muscarinic toxins.

    • Organizational Affiliation: 

    CEA, DSV, iBiTec-S, Service d'Ingénierie Moléculaire des Protéines, 91191 Gif-sur-Yvette, France.


Macromolecules
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(by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Toxin AdTx166Dendroaspis angusticepsMutation(s): 1 
UniProt
Find proteins for P85092 (Dendroaspis angusticeps)
Explore P85092 
Go to UniProtKB:  P85092
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP85092
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.95 Å
  • R-Value Free: 0.252 
  • R-Value Work: 0.170 
  • R-Value Observed: 0.174 
  • Space Group: P 31 2 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 37.37α = 90
b = 37.37β = 90
c = 66.05γ = 120
Software Package:
Software NamePurpose
DNAdata collection
MOLREPphasing
REFMACrefinement
XDSdata reduction
XDSdata scaling

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2013-05-08
    Type: Initial release
  • Version 1.1: 2013-08-28
    Changes: Database references
  • Version 1.2: 2023-09-20
    Changes: Data collection, Database references, Derived calculations, Refinement description