Download MendeleyCrystal structure of a putative Cytosolic malate dehydrogenase from Leishmania major Friedlin in complex with NAD
SSGCID, Abendroth, J., Edwards, T.E., Myler, P.To be published.
4I1I
Crystal structure of a putative Cytosolic malate dehydrogenase from Leishmania major Friedlin in complex with NAD
- PDB DOI: https://doi.org/10.2210/pdb4I1I/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Leishmania major strain Friedlin
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2012-11-21 Released: 2012-12-19
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.50 Å
- R-Value Free: 0.166
- R-Value Work: 0.145
- R-Value Observed: 0.146
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Malate dehydrogenase | 345 | Leishmania major strain Friedlin | Mutation(s): 0 Gene Names: cMDH, LMJF_28_2860 EC: 1.1.1.37 |  | |
UniProt | |||||
Find proteins for Q4Q7X6 (Leishmania major) Explore Q4Q7X6 Go to UniProtKB: Q4Q7X6 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q4Q7X6 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| NAD Query on NAD | C [auth A] | NICOTINAMIDE-ADENINE-DINUCLEOTIDE C21 H27 N7 O14 P2 BAWFJGJZGIEFAR-NNYOXOHSSA-N |  | ||
| PO4 Query on PO4 | D [auth A], I [auth B], J [auth B] | PHOSPHATE ION O4 P NBIIXXVUZAFLBC-UHFFFAOYSA-K |  | ||
| EDO Query on EDO | E [auth A], F [auth A], G [auth A], H [auth A], K [auth B] | 1,2-ETHANEDIOL C2 H6 O2 LYCAIKOWRPUZTN-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.50 Å
- R-Value Free: 0.166
- R-Value Work: 0.145
- R-Value Observed: 0.146
- Space Group: P 1 21 1
- Diffraction Data: https://doi.org/10.18430/M34I1I
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 64.82 | α = 90 |
| b = 65.13 | β = 110.67 |
| c = 77.49 | γ = 90 |
| Software Name | Purpose |
|---|---|
| XSCALE | data scaling |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| XDS | data reduction |
| REFMAC | phasing |
Entry History
Deposition Data
- Released Date: 2012-12-19 Deposition Author(s): Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)
Revision History (Full details and data files)
- Version 1.0: 2012-12-19
Type: Initial release - Version 1.1: 2017-11-15
Changes: Refinement description - Version 1.2: 2023-09-20
Changes: Data collection, Database references, Derived calculations, Refinement description
