4FFV

Crystal Structure of Dipeptidyl Peptidase IV (DPP4, DPP-IV, CD26) in Complex with 11A19 Fab

PDB DOI: https://doi.org/10.2210/pdb4FFV/pdb

Classification: HYDROLASE/Immune System, INHIBITOR
Organism(s): Rattus norvegicus, Mus musculus
Expression System: Homo sapiens
Mutation(s): No

Deposited: 2012-06-01 Released: 2012-12-12
Deposition Author(s): Wang, Z., Sudom, A., Walker, N.P., Min, X.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.40 Å
R-Value Free: 0.301
R-Value Work: 0.271
R-Value Observed: 0.272

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

An Inhibitory Antibody Against DPP IV Improves Glucose Tolerance in vivo - Validation of Large Molecule Approach for DPP IV Inhibition

Tang, J., Majeti, J., Sudom, A., Xiong, Y., Lu, M., Liu, Q., Higbee, J., Zhang, Y., Wang, Y., Wang, W., Cao, P., Xia, Z., Johnstone, S., Yang, X., Yu, T., Sharkov, N., Walker, N., Tu, H., Shen, W., Wang, Z., Min, X.

To be published.

Macromolecule Content

Total Structure Weight: 261.02 kDa
Atom Count: 18,423
Modelled Residue Count: 2,288
Deposited Residue Count: 2,314
Unique protein chains: 3

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Dipeptidyl peptidase 4	A, B	730	Rattus norvegicus	Mutation(s): 0 Gene Names: Dpp4, Cd26 EC: 3.4.14.5
UniProt
Find proteins for P14740 (Rattus norvegicus) Explore P14740 Go to UniProtKB: P14740
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P14740
Sequence Annotations Expand
Reference Sequence

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Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
11A19 Fab light chain	C, E [auth L]	210	Mus musculus	Mutation(s): 0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations Expand
Reference Sequence

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(by identity cutoff) | 3D Structure

Entity ID: 3
Molecule	Chains	Sequence Length	Organism	Details	Image
11A19 Fab heavy chain	D, F [auth H]	217	Mus musculus	Mutation(s): 0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.40 Å
R-Value Free: 0.301
R-Value Work: 0.271
R-Value Observed: 0.272
Space Group: P 1 2₁ 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 97.26	α = 90
b = 201.72	β = 93.71
c = 97.28	γ = 90

Software Package:

Software Name	Purpose
MOSFLM	data reduction
SCALA	data scaling
REFMAC	refinement
PDB_EXTRACT	data extraction

Structure Validation

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Entry History

Deposition Data

Released Date: 2012-12-12

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2012-12-12
Type: Initial release
Version 1.1: 2021-05-19
Changes: Source and taxonomy

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.