4FFH
Crystal Structure of Levan Fructotransferase D54N mutant from Arthrobacter ureafaciens in complex with sucrose
- PDB DOI: https://doi.org/10.2210/pdb4FFH/pdb
- Classification: TRANSFERASE
- Organism(s): Paenarthrobacter ureafaciens
- Expression System: Escherichia coli
- Mutation(s): Yes 
- Deposited: 2012-06-01 Released: 2012-07-18 
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.218 
- R-Value Work: 0.190 
wwPDB Validation   3D Report Full Report
This is version 2.1 of the entry. See complete history. 
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
Levan fructotransferase | 492 | Paenarthrobacter ureafaciens | Mutation(s): 1  Gene Names: lftA EC: 4.2.2.16 | ||
UniProt | |||||
Find proteins for Q9KJD0 (Paenarthrobacter ureafaciens) Explore Q9KJD0  Go to UniProtKB:  Q9KJD0 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | Q9KJD0 | ||||
Sequence AnnotationsExpand | |||||
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Oligosaccharides
Biologically Interesting Molecules (External Reference) 1 Unique
Entity ID: 2 | |||||
---|---|---|---|---|---|
ID | Chains | Name | Type/Class | 2D Diagram | 3D Interactions |
PRD_900003 Query on PRD_900003 | E, F, G, H, I | sucrose | Oligosaccharide / Nutrient |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.218 
- R-Value Work: 0.190 
- Space Group: P 21 21 21
Unit Cell:
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 82.284 | α = 90 |
b = 167.004 | β = 90 |
c = 261.926 | γ = 90 |
Software Name | Purpose |
---|---|
CNS | refinement |
PDB_EXTRACT | data extraction |
HKL-2000 | data collection |
HKL-2000 | data reduction |
HKL-2000 | data scaling |
Entry History 
Revision History (Full details and data files)
- Version 1.0: 2012-07-18
Type: Initial release - Version 1.1: 2012-08-29
Changes: Database references - Version 1.2: 2012-09-26
Changes: Database references - Version 1.3: 2017-05-10
Changes: Non-polymer description - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Database references, Derived calculations, Non-polymer description, Structure summary - Version 2.1: 2024-02-28
Changes: Data collection, Database references, Structure summary