4EXK
A chimera protein containing MBP fused to the C-terminal domain of the uncharacterized protein STM14_2015 from Salmonella enterica
- PDB DOI: https://doi.org/10.2210/pdb4EXK/pdb
- Classification: TRANSPORT PROTEIN
- Organism(s): Escherichia coli K-12, Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes 
- Deposited: 2012-04-30 Released: 2012-08-08 
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.28 Å
- R-Value Free: 0.178 
- R-Value Work: 0.141 
- R-Value Observed: 0.143 
wwPDB Validation   3D Report Full Report
This is version 2.1 of the entry. See complete history. 
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
Maltose-binding periplasmic protein, uncharacterized protein chimera | 487 | Escherichia coli K-12, Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S This entity is chimeric | Mutation(s): 9  Gene Names: malE, b4034, JW3994, STM14_2015, STM1668 | ||
UniProt | |||||
Find proteins for P0AEX9 (Escherichia coli (strain K12)) Explore P0AEX9  Go to UniProtKB:  P0AEX9 | |||||
Find proteins for A0A0F6B1U8 (Salmonella typhimurium (strain 14028s / SGSC 2262)) Explore A0A0F6B1U8  Go to UniProtKB:  A0A0F6B1U8 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Groups | P0AEX9A0A0F6B1U8 | ||||
Sequence AnnotationsExpand | |||||
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Oligosaccharides
Small Molecules
Ligands 1 Unique | |||||
---|---|---|---|---|---|
ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
PGE Query on PGE | C [auth A] | TRIETHYLENE GLYCOL C6 H14 O4 ZIBGPFATKBEMQZ-UHFFFAOYSA-N |
Biologically Interesting Molecules (External Reference) 1 Unique
Entity ID: 2 | |||||
---|---|---|---|---|---|
ID | Chains | Name | Type/Class | 2D Diagram | 3D Interactions |
PRD_900010 Query on PRD_900010 | B | alpha-maltotetraose | Oligosaccharide / Substrate analog |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.28 Å
- R-Value Free: 0.178 
- R-Value Work: 0.141 
- R-Value Observed: 0.143 
- Space Group: P 32 2 1
- Diffraction Data: https://doi.org/10.18430/M34EXK
Unit Cell:
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 64.079 | α = 90 |
b = 64.079 | β = 90 |
c = 180.912 | γ = 120 |
Software Name | Purpose |
---|---|
SBC-Collect | data collection |
MOLREP | phasing |
REFMAC | refinement |
HKL-3000 | data reduction |
HKL-3000 | data scaling |
Entry History 
Deposition Data
- Released Date: 2012-08-08  Deposition Author(s): Nocek, B., Hatzos-Skintges, C., Jedrzejczak, R., Babnigg, G., Brown, R.N., Cort, J.R., Heffron, F., Nakayasu, E.S., Adkins, J.N., Joachimiak, A., Program for the Characterization of Secreted Effector Proteins (PCSEP), Midwest Center for Structural Genomics (MCSG)
Revision History (Full details and data files)
- Version 1.0: 2012-08-08
Type: Initial release - Version 1.1: 2012-08-22
Changes: Database references, Structure summary - Version 1.2: 2017-08-16
Changes: Refinement description, Source and taxonomy - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Database references, Derived calculations, Non-polymer description, Structure summary - Version 2.1: 2023-09-13
Changes: Data collection, Database references, Refinement description, Structure summary