Download MendeleyThe crystal structure of beta-lactamase from Sphaerobacter thermophilus DSM 20745
Wu, R., Clancy, S., Joachimiak, A.To be published.
4EWF
The crystal structure of beta-lactamase from Sphaerobacter thermophilus DSM 20745
- PDB DOI: https://doi.org/10.2210/pdb4EWF/pdb
- Classification: HYDROLASE
- Organism(s): Sphaerobacter thermophilus DSM 20745
- Expression System: Escherichia coli BL21
- Mutation(s): No
- Deposited: 2012-04-26 Released: 2012-09-19
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.209
- R-Value Work: 0.181
- R-Value Observed: 0.182
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Beta-lactamase | 275 | Sphaerobacter thermophilus DSM 20745 | Mutation(s): 0 Gene Names: Sthe_2032 |  | |
UniProt | |||||
Find proteins for D1C5R0 (Sphaerobacter thermophilus (strain DSM 20745 / S 6022)) Explore D1C5R0 Go to UniProtKB: D1C5R0 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | D1C5R0 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SO4 Query on SO4 | E [auth A], F [auth A], G [auth B], H [auth C], J [auth D] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| ACY Query on ACY | I [auth C] | ACETIC ACID C2 H4 O2 QTBSBXVTEAMEQO-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A, B, C, D | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.209
- R-Value Work: 0.181
- R-Value Observed: 0.182
- Space Group: C 2 2 21
- Diffraction Data: https://doi.org/10.18430/M34EWF
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 137.5 | α = 90 |
| b = 215.551 | β = 90 |
| c = 83.816 | γ = 90 |
| Software Name | Purpose |
|---|---|
| HKL-3000 | data collection |
| MLPHARE | phasing |
| PHENIX | refinement |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
Entry History
Deposition Data
- Released Date: 2012-09-19 Deposition Author(s): Wu, R., Clancy, S., Joachimiak, A., Midwest Center for Structural Genomics (MCSG)
Revision History (Full details and data files)
- Version 1.0: 2012-09-19
Type: Initial release
