4CRO

PROTEIN-DNA CONFORMATIONAL CHANGES IN THE CRYSTAL STRUCTURE OF A LAMBDA CRO-OPERATOR COMPLEX


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 3.90 Å

wwPDB Validation   3D Report Full Report


This is version 2.0 of the entry. See complete history


Literature

Protein-DNA conformational changes in the crystal structure of a lambda Cro-operator complex.

Brennan, R.G.Roderick, S.L.Takeda, Y.Matthews, B.W.

(1990) Proc Natl Acad Sci U S A 87: 8165-8169

  • DOI: https://doi.org/10.1073/pnas.87.20.8165
  • Primary Citation of Related Structures:  
    4CRO

  • PubMed Abstract: 

    The structure of a complex of bacteriophage lambda Cro protein with a 17-base-pair operator has been determined at 3.9-A resolution. Isomorphous derivatives obtained by the synthesis of site-specific iodinated DNA oligomers were of critical importance in solving the structure. The crystal structure contains three independent Cro-operator complexes that have very similar, although not necessarily identical, conformations. In the complex, the protein dimer undergoes a large conformational change relative to the crystal structure of the free protein. One monomer rotates by about 40 degrees relative to the other, this being accomplished primarily by a twisting of the two beta-sheet strands that connect one monomer with the other. In the complex, the DNA is bent by about 40 degrees into the shape of a boomerang but maintains essentially Watson-Crick B-form. In contrast to other known protein-DNA complexes, the DNA is not stacked end-to-end. The structure confirms the general features of the model previously proposed for the interaction of Cro with DNA.


  • Organizational Affiliation

    Department of Physics, University of Oregon, Eugene 97403.


Macromolecules

Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
PROTEIN (LAMBDA CRO)66Lambdavirus lambdaMutation(s): 0 
UniProt
Find proteins for P03040 (Escherichia phage lambda)
Explore P03040 
Go to UniProtKB:  P03040
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP03040
Sequence Annotations
Expand
  • Reference Sequence

Find similar nucleic acids by:  Sequence   |   3D Structure  

Entity ID: 1
MoleculeChains LengthOrganismImage
DNA (5'-D(*TP*AP*TP*CP*AP*CP*CP*GP*CP*GP*GP*GP*TP*GP*AP*TP*A)-3')17N/A
Sequence Annotations
Expand
  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 3.90 Å
  • Space Group: P 32
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 154.8α = 90
b = 154.8β = 90
c = 86γ = 120

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 1992-01-15
    Type: Initial release
  • Version 1.1: 2008-05-22
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2020-07-22
    Changes: Author supporting evidence, Data collection, Other, Refinement description
  • Version 2.0: 2022-11-23
    Type: Remediation
    Changes: Advisory, Atomic model, Author supporting evidence, Data collection, Database references, Refinement description