Download MendeleyThe Ymdb Phosphodiesterase is a Global Regulator of Late Adaptive Responses in Bacillus Subtilis.
Diethmaier, C., Newman, J.A., Kovacs, A.T., Kaever, V., Herzberg, C., Rodrigues, C., Boonstra, M., Kuipers, O.P., Lewis, R.J., Stulke, J.(2014) J Bacteriol 196: 265
- PubMed: 24163345
- DOI: https://doi.org/10.1128/JB.00826-13
- Primary Citation of Related Structures:
4B2O - PubMed Abstract:
Bacillus subtilis mutants lacking ymdB are unable to form biofilms, exhibit a strong overexpression of the flagellin gene hag, and are deficient in SlrR, a SinR antagonist. Here, we report the functional and structural characterization of YmdB, and we find that YmdB is a phosphodiesterase with activity against 2',3'- and 3',5'-cyclic nucleotide monophosphates. The structure of YmdB reveals that the enzyme adopts a conserved phosphodiesterase fold with a binuclear metal center. Mutagenesis of a catalytically crucial residue demonstrates that the enzymatic activity of YmdB is essential for biofilm formation. The deletion of ymdB affects the expression of more than 800 genes; the levels of the σ(D)-dependent motility regulon and several sporulation genes are increased, and the levels of the SinR-repressed biofilm genes are decreased, confirming the role of YmdB in regulating late adaptive responses of B. subtilis.
Organizational Affiliation:
Department of General Microbiology, Georg August University Göttingen, Göttingen, Germany.
