486D

X-RAY CRYSTAL STRUCTURES OF 70S RIBOSOME FUNCTIONAL COMPLEXES


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 7.50 Å

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This is version 1.3 of the entry. See complete history


Literature

X-ray crystal structures of 70S ribosome functional complexes.

Cate, J.H.Yusupov, M.M.Yusupova, G.Z.Earnest, T.N.Noller, H.F.

(1999) Science 285: 2095-2104

  • DOI: https://doi.org/10.1126/science.285.5436.2095
  • Primary Citation of Related Structures:  
    486D

  • PubMed Abstract: 

    Structures of 70S ribosome complexes containing messenger RNA and transfer RNA (tRNA), or tRNA analogs, have been solved by x-ray crystallography at up to 7.8 angstrom resolution. Many details of the interactions between tRNA and the ribosome, and of the packing arrangements of ribosomal RNA (rRNA) helices in and between the ribosomal subunits, can be seen. Numerous contacts are made between the 30S subunit and the P-tRNA anticodon stem-loop; in contrast, the anticodon region of A-tRNA is much more exposed. A complex network of molecular interactions suggestive of a functional relay is centered around the long penultimate stem of 16S rRNA at the subunit interface, including interactions involving the "switch" helix and decoding site of 16S rRNA, and RNA bridges from the 50S subunit.


  • Organizational Affiliation

    Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064, USA. cate@wi.mit.edu


Macromolecules
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Entity ID: 1
MoleculeChains LengthOrganismImage
P-SITE TRNA OF 70S RIBOSOME74Escherichia coli
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Entity ID: 2
MoleculeChains LengthOrganismImage
P-SITE CODON OF 70S RIBOSOME3N/A
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Entity ID: 3
MoleculeChains LengthOrganismImage
A-SITE TRNA OF 70S RIBOSOME72Saccharomyces cerevisiae
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Entity ID: 4
MoleculeChains LengthOrganismImage
A-SITE CODON OF 70S RIBOSOME3N/A
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Entity ID: 5
MoleculeChains LengthOrganismImage
E-SITE TRNA OF 70S RIBOSOME72Thermus thermophilus
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Entity ID: 6
MoleculeChains LengthOrganismImage
PENULTIMATE STEM OF 16S RRNA IN THE 70S RIBOSOME85Thermus thermophilus
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Entity ID: 7
MoleculeChains LengthOrganismImage
900 STEM-LOOP OF 16S RRNA IN THE 70S RIBOSOME33Thermus thermophilus
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 7.50 Å
  • Space Group: I 4 2 2
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 508.02α = 90
b = 508.02β = 90
c = 802.79γ = 90
Software Package:
Software NamePurpose
MLPHAREphasing
Omodel building
DENZOdata reduction
SCALEPACKdata scaling

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 1999-10-04
    Type: Initial release
  • Version 1.1: 2008-04-27
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Non-polymer description, Version format compliance
  • Version 1.3: 2023-09-13
    Changes: Data collection, Database references, Derived calculations, Refinement description