3ZMI
Structure of E.coli rhomboid protease GlpG in complex with monobactam L29
- PDB DOI: https://doi.org/10.2210/pdb3ZMI/pdb
- Classification: HYDROLASE
- Organism(s): Escherichia coli
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Membrane Protein: Yes PDBTMMemProtMD
- Deposited: 2013-02-11 Released: 2013-05-22
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.261
- R-Value Work: 0.216
- R-Value Observed: 0.219
This is version 1.5 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| RHOMBOID PROTEASE GLPG | 179 | Escherichia coli | Mutation(s): 0 EC: 3.4.21.105 Membrane Entity: Yes |  | |
UniProt | |||||
Find proteins for P09391 (Escherichia coli (strain K12)) Explore P09391 Go to UniProtKB: P09391 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P09391 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| BNG Query on BNG | C [auth A] D [auth A] E [auth A] F [auth A] G [auth A] | nonyl beta-D-glucopyranoside C15 H30 O6 QFAPUKLCALRPLH-UXXRCYHCSA-N |  | ||
| L6C Query on L6C | B [auth A] | phenyl N-[(1R)-3-oxidanylidene-1-phenyl-propyl]carbamate C16 H15 N O3 HHWCOMIRIZKFCW-OAHLLOKOSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.261
- R-Value Work: 0.216
- R-Value Observed: 0.219
- Space Group: H 3 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 110.58 | α = 90 |
| b = 110.58 | β = 90 |
| c = 128.53 | γ = 120 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| MOSFLM | data reduction |
| SCALA | data scaling |
| PHASER | phasing |
Entry History
Deposition Data
- Released Date: 2013-05-22 Deposition Author(s): Vinothkumar, K.R., Pierrat, O.A., Large, J.M., Freeman, M.
Revision History (Full details and data files)
- Version 1.0: 2013-05-22
Type: Initial release - Version 1.1: 2013-05-29
Changes: Database references - Version 1.2: 2013-08-28
Changes: Database references - Version 1.3: 2019-02-27
Changes: Data collection, Database references, Derived calculations, Experimental preparation, Other - Version 1.4: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Derived calculations, Other, Structure summary - Version 1.5: 2023-12-20
Changes: Data collection, Database references, Refinement description, Structure summary
