Cryo-EM structure of the F420-reducing NiFe-hydrogenase from a methanogenic archaeon with bound substrate
3ZFS
Primary Citation
 
 
  •   Molecular Description Hide
    Classification: Oxidoreductase
    Structure Weight: 109136.71
    Molecule: F420-REDUCING HYDROGENASE, SUBUNIT ALPHA
    Polymer: 1 Type: protein Length: 405
    Chains: A
    EC#: 1.12.98.1   
    Details: FRHA CONTAINS A NIFE CENTER
    Organism: Methanothermobacter marburgensis
    Gene Names: frhA MTBMA_c16860
    UniProtKB: Protein Feature View | Search PDB | D9PYF9  
    Molecule: F420-REDUCING HYDROGENASE, SUBUNIT GAMMA
    Polymer: 2 Type: protein Length: 275
    Chains: B
    EC#: 1.12.98.1   
    Details: FRHG CONTAINS THREE 4FE4S CLUSTERS
    Organism: Methanothermobacter marburgensis
    Gene Names: frhG MTBMA_c16840
    UniProtKB: Protein Feature View | Search PDB | D9PYF7  
    Molecule: F420-REDUCING HYDROGENASE, SUBUNIT BETA
    Polymer: 3 Type: protein Length: 281
    Chains: C
    EC#: 1.12.98.1   
    Details: FRHB CONTAINS FAD AND A 4FE4S CLUSTER
    Organism: Methanothermobacter marburgensis
    Gene Names: frhB MTBMA_c16830
    UniProtKB: Protein Feature View | Search PDB | D9PYF6  
     

  •   Related Citations in PDB Entry (REMARK 1) Hide
     
  •   Ligand Chemical Component Hide
    Identifier Formula Name View Interactions
    F42
    Search 
    Download 
    F42 C29 H36 N5 O18 P
    COENZYME F420
    FAD
    Search 
    Download 
    FAD C27 H33 N9 O15 P2
    FLAVIN-ADENINE DINUCLEOTIDE
    FCO
    Search 
    Download 
    FCO C3 Fe N2 O
    CARBONMONOXIDE-(DICYANO) IRON
    FE2
    Search 
    Download 
    FE2 Fe
    FE (II) ION
    NI
    Search 
    Download 
    NI Ni
    NICKEL (II) ION
    SF4
    Search 
    Download 
    SF4 Fe4 S4
    IRON/SULFUR CLUSTER
     
  •   External Domain Annotations Hide
     
  •   Structural Biology Knowledgebase Data Hide
     
 
Data in orange boxes are gathered from external resources (when available).
  Biological Assembly       
Biological assembly 1 assigned by authors
Downloadable viewers:
 
 
 
  •   MyPDB Personal Annotations Hide
     
  •   Deposition Summary Hide
    Authors:   Mills, D.J.,  Vitt, S.,  Strauss, M.,  Shima, S.,  Vonck, J.

    Deposition:   2012-12-12
    Release:   2013-03-06
    Last Modified (REVDAT):   2013-04-03
     
  •   Revision History    Hide
    Mouse over text for details
    2013-04-03
    Citation
     
  •   Experimental Details Hide
    Method:   ELECTRON MICROSCOPY
    Exp. Data:


    EM Resolution [Å]: 4.0
    Aggregation State: PARTICLE
    Reconstruction Method: SINGLE PARTICLE
    Specimen Type: VITREOUS ICE - CRYO EM