Download MendeleyAnion tags improve extracellular production of Candida antarctica lipase B in Escherichia coli
Kim, S.K., Lee, H.H., Park, Y.C., Jeon, S.T., Son, S.H., Min, W.K., Seo, J.H.To be published.
3W9B
Crystal structure of Candida antarctica lipase B with anion-tag
- PDB DOI: https://doi.org/10.2210/pdb3W9B/pdb
- Classification: HYDROLASE
- Organism(s): Moesziomyces antarcticus
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2013-04-02 Released: 2014-04-02
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.90 Å
- R-Value Free: 0.265
- R-Value Work: 0.232
- R-Value Observed: 0.234
wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Lipase B | 328 | Moesziomyces antarcticus | Mutation(s): 0 EC: 3.1.1.3 |  | |
UniProt | |||||
Find proteins for P41365 (Pseudozyma antarctica) Explore P41365 Go to UniProtKB: P41365 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P41365 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PE8 Query on PE8 | E [auth A], F [auth B], G [auth C], H [auth D] | 3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL C16 H34 O9 GLZWNFNQMJAZGY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.90 Å
- R-Value Free: 0.265
- R-Value Work: 0.232
- R-Value Observed: 0.234
- Space Group: P 65
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 123.659 | α = 90 |
| b = 123.659 | β = 90 |
| c = 191.852 | γ = 120 |
| Software Name | Purpose |
|---|---|
| HKL-2000 | data collection |
| PHASER | phasing |
| REFMAC | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
Entry History
Deposition Data
- Released Date: 2014-04-02 Deposition Author(s): Kim, S.K., Lee, H.H., Park, Y.C., Jeon, S.T., Son, S.H., Min, W.K., Seo, J.H.
Revision History (Full details and data files)
- Version 1.0: 2014-04-02
Type: Initial release - Version 1.1: 2023-11-08
Changes: Data collection, Database references, Derived calculations, Refinement description
