Download MendeleyCueO E506D Mutant: Crystal Structure of Reduced Native Intermediate, Kinetics, and Impairment of Product Release
Singh, S.K., Roberts, S.A., Hazzard, J.T., Franke-McDevitt, S., Weichsel, A., Rensing, C., Montfort, W.R.To be published.
3QQX
Reduced Native Intermediate of the Multicopper Oxidase CueO
- PDB DOI: https://doi.org/10.2210/pdb3QQX/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Escherichia coli K-12
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2011-02-16 Released: 2012-02-29
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.50 Å
- R-Value Free: 0.242
- R-Value Work: 0.206
- R-Value Observed: 0.208
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Blue copper oxidase CueO | 505 | Escherichia coli K-12 | Mutation(s): 1 Gene Names: b0123, cueO, JW0119, yacK |  | |
UniProt | |||||
Find proteins for P36649 (Escherichia coli (strain K12)) Explore P36649 Go to UniProtKB: P36649 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P36649 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| CU1 Query on CU1 | D [auth A], E [auth A], F [auth A], G [auth A], H [auth A] | COPPER (I) ION Cu VMQMZMRVKUZKQL-UHFFFAOYSA-N |  | ||
| CU Query on CU | B [auth A], C [auth A], I [auth A] | COPPER (II) ION Cu JPVYNHNXODAKFH-UHFFFAOYSA-N |  | ||
| O Query on O | J [auth A], K [auth A] | OXYGEN ATOM O XLYOFNOQVPJJNP-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.50 Å
- R-Value Free: 0.242
- R-Value Work: 0.206
- R-Value Observed: 0.208
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 50.173 | α = 90 |
| b = 91.375 | β = 102.22 |
| c = 54.328 | γ = 90 |
| Software Name | Purpose |
|---|---|
| CrystalClear | data collection |
| REFMAC | refinement |
| CrystalClear | data reduction |
| CrystalClear | data scaling |
Entry History
Deposition Data
- Released Date: 2012-02-29 Deposition Author(s): Montfort, W.R., Roberts, S.A., Singh, S.K.
Revision History (Full details and data files)
- Version 1.0: 2012-02-29
Type: Initial release - Version 1.1: 2017-11-08
Changes: Refinement description - Version 1.2: 2023-09-13
Changes: Data collection, Database references, Derived calculations, Refinement description
