3P08

Crystal structure of the human BTK kinase domain

PDB DOI: https://doi.org/10.2210/pdb3P08/pdb

Classification: TRANSFERASE
Organism(s): Homo sapiens
Expression System: Spodoptera frugiperda
Mutation(s): No

Deposited: 2010-09-27 Released: 2010-11-03
Deposition Author(s): Yu, C.L., Hymowitz, S.G.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.30 Å
R-Value Free: 0.278
R-Value Work: 0.222
R-Value Observed: 0.228

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

A novel, specific BTK inhibitor antagonizes BCR and FcgR signaling and suppresses inflammatory arthritis

Di Paolo, J., Huang, T., Balazs, M., Barbosa, J., Barck, K.H., Bravo, B., Carano, R.A.D., Darrow, J., Davies, D.R., DeForge, L.E., Diehl, L., Ferrando, R., Gallion, S.L., Gianetti, A.M., Gribling, P., Hurez, V., Hymowitz, S.G., Jones, R., Kropf, J.E., Lee, W.P., Maciejewski, P.M., Mitchell, S.A., Rong, H., Staker, B.L., Whitney, J.A., Yeh, S., Young, W., Yu, C., Zhang, J., Reif, K., Currie, K.S.

To be published.

Macromolecule Content

Total Structure Weight: 62.42 kDa
Atom Count: 4,335
Modelled Residue Count: 510
Deposited Residue Count: 534
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Tyrosine-protein kinase BTK	A, B	267	Homo sapiens	Mutation(s): 0 Gene Names: AGMX1, ATK, BPK, BTK EC: 2.7.10.2
UniProt & NIH Common Fund Data Resources
Find proteins for Q06187 (Homo sapiens) Explore Q06187 Go to UniProtKB: Q06187
PHAROS: Q06187 GTEx: ENSG00000010671
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q06187
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.30 Å
R-Value Free: 0.278
R-Value Work: 0.222
R-Value Observed: 0.228
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 46.602	α = 90
b = 103.493	β = 90
c = 117.564	γ = 90

Software Package:

Software Name	Purpose
BOS	data collection
PHASER	phasing
REFMAC	refinement
HKL-2000	data reduction
SCALEPACK	data scaling

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2010-11-03

Deposition Author(s):

Yu, C.L.

Hymowitz, S.G.

Revision History (Full details and data files)

Version 1.0: 2010-11-03
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance
Version 1.2: 2018-01-24
Changes: Database references
Version 1.3: 2023-09-06
Changes: Data collection, Database references, Refinement description

Prepare Data

Validate Data

Deposit Data

Help and Resources

3P08

Crystal structure of the human BTK kinase domain

A novel, specific BTK inhibitor antagonizes BCR and FcgR signaling and suppresses inflammatory arthritis

Entity ID: 1

UniProt & NIH Common Fund Data Resources

Entity Groups

Sequence Annotations

Expand

Experimental Data

Structure Validation

Deposition Data

Revision History (Full details and data files)