3OW5

Crystal structure of the Y200A mutant of gamma carbonic anhydrase from Methanosarcina thermophila


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.80 Å
  • R-Value Free: 0.152 
  • R-Value Work: 0.129 
  • R-Value Observed: 0.130 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history


Literature

Crystal structure of the Y200A mutant of Methanosarcina thermophila

Domsic, J.F.Robbins, A.H.McKenna, R.

To be published.

Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Carbonic anhydrase213Methanosarcina thermophilaMutation(s): 1 
EC: 4.2.1.1
UniProt
Find proteins for P40881 (Methanosarcina thermophila (strain ATCC 43570 / DSM 1825 / OCM 12 / VKM B-1830 / TM-1))
Explore P40881 
Go to UniProtKB:  P40881
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP40881
Sequence Annotations
Expand
  • Reference Sequence
Small Molecules
Binding Affinity Annotations 
IDSourceBinding Affinity
SO4 BindingDB:  3OW5 Ki: min: 1.17e+7, max: 2.00e+8 (nM) from 2 assay(s)
BCT BindingDB:  3OW5 Ki: min: 6.70e+6, max: 4.20e+7 (nM) from 2 assay(s)
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.80 Å
  • R-Value Free: 0.152 
  • R-Value Work: 0.129 
  • R-Value Observed: 0.130 
  • Space Group: P 21 3
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 83.498α = 90
b = 83.498β = 90
c = 83.498γ = 90
Software Package:
Software NamePurpose
DENZOdata reduction
SCALEPACKdata scaling
PHENIXrefinement
PDB_EXTRACTdata extraction
CrystalCleardata collection
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2011-10-26
    Type: Initial release
  • Version 1.1: 2017-11-08
    Changes: Refinement description
  • Version 1.2: 2024-02-21
    Changes: Data collection, Database references, Derived calculations