Download MendeleyCrystal Structure of tRNA/rRNA Methyltransferase SpoU from Rhodobacter sphaeroides
Kim, Y., Tesar, C., Jedrzejczak, R., Joachimiak, A.To be published.
3ONP
Crystal Structure of tRNA/rRNA Methyltransferase SpoU from Rhodobacter sphaeroides
- PDB DOI: https://doi.org/10.2210/pdb3ONP/pdb
- Classification: TRANSFERASE
- Organism(s): Cereibacter sphaeroides 2.4.1
- Expression System: Escherichia coli BL21
- Mutation(s): No
- Deposited: 2010-08-30 Released: 2010-09-08
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.90 Å
- R-Value Free: 0.219
- R-Value Work: 0.189
- R-Value Observed: 0.191
wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| tRNA/rRNA methyltransferase (SpoU) | 249 | Cereibacter sphaeroides 2.4.1 | Mutation(s): 0 Gene Names: RHOS4_30450, RSP_3829 |  | |
UniProt | |||||
Find proteins for Q3IXX1 (Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.)) Explore Q3IXX1 Go to UniProtKB: Q3IXX1 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q3IXX1 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 4 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| GOL Query on GOL | D [auth A] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
| BME Query on BME | C [auth A], F [auth A] | BETA-MERCAPTOETHANOL C2 H6 O S DGVVWUTYPXICAM-UHFFFAOYSA-N |  | ||
| ACY Query on ACY | B [auth A], G [auth A] | ACETIC ACID C2 H4 O2 QTBSBXVTEAMEQO-UHFFFAOYSA-N |  | ||
| CL Query on CL | E [auth A] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.90 Å
- R-Value Free: 0.219
- R-Value Work: 0.189
- R-Value Observed: 0.191
- Space Group: P 65 2 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 74.782 | α = 90 |
| b = 74.782 | β = 90 |
| c = 139.105 | γ = 120 |
| Software Name | Purpose |
|---|---|
| SBC-Collect | data collection |
| HKL-3000 | data collection |
| HKL-3000 | phasing |
| SHELXS | phasing |
| MLPHARE | phasing |
| RESOLVE | model building |
| SOLVE | phasing |
| REFMAC | refinement |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
| RESOLVE | phasing |
Entry History
Deposition Data
- Released Date: 2010-09-08 Deposition Author(s): Kim, Y., Tesar, C., Jedrzejczak, R., Joachimiak, A., Midwest Center for Structural Genomics (MCSG)
Revision History (Full details and data files)
- Version 1.0: 2010-09-08
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance
