Download Mendeley2.0 Angstrom Crystal structure of Glutamate--Cysteine Ligase (gshA) ftom Francisella tularensis in Complex with AMP.
Minasov, G., Halavaty, A., Shuvalova, L., Dubrovska, I., Winsor, J., Papazisi, L., Anderson, W.F.To be published.
3NZT
2.0 Angstrom Crystal structure of Glutamate--Cysteine Ligase (gshA) ftom Francisella tularensis in Complex with AMP
- PDB DOI: https://doi.org/10.2210/pdb3NZT/pdb
- Classification: LIGASE
- Organism(s): Francisella tularensis subsp. tularensis
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2010-07-16 Released: 2010-07-28
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.196
- R-Value Work: 0.164
- R-Value Observed: 0.165
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Glutamate--cysteine ligase | 525 | Francisella tularensis subsp. tularensis | Mutation(s): 0 Gene Names: FTT_0367c, gshA EC: 6.3.2.2 |  | |
UniProt | |||||
Find proteins for Q5NHS8 (Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)) Explore Q5NHS8 Go to UniProtKB: Q5NHS8 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q5NHS8 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| AMP Query on AMP | B [auth A] | ADENOSINE MONOPHOSPHATE C10 H14 N5 O7 P UDMBCSSLTHHNCD-KQYNXXCUSA-N |  | ||
| SO4 Query on SO4 | C [auth A] D [auth A] E [auth A] F [auth A] G [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.196
- R-Value Work: 0.164
- R-Value Observed: 0.165
- Space Group: P 65
- Diffraction Data: https://doi.org/10.18430/M33NZT
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 89.849 | α = 90 |
| b = 89.849 | β = 90 |
| c = 151.468 | γ = 120 |
| Software Name | Purpose |
|---|---|
| Blu-Ice | data collection |
| PHASER | phasing |
| REFMAC | refinement |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
Entry History
Deposition Data
- Released Date: 2010-07-28 Deposition Author(s): Minasov, G., Halavaty, A., Shuvalova, L., Dubrovska, I., Winsor, J., Papazisi, L., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)
Revision History (Full details and data files)
- Version 1.0: 2010-07-28
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2017-11-08
Changes: Advisory, Refinement description - Version 1.3: 2023-09-06
Changes: Advisory, Data collection, Database references, Derived calculations, Refinement description
