3NJC

Crystal structure of the yslB protein from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR460.

PDB DOI: https://doi.org/10.2210/pdb3NJC/pdb

Classification: structural genomics, unknown function
Organism(s): Bacillus subtilis subsp. subtilis str. 168
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2010-06-17 Released: 2010-09-22
Deposition Author(s): Vorobiev, S., Abashidze, M., Seetharaman, J., Wang, D., Cunningham, K., Ma, L.-C., Owens, L., Fang, Y., Xiao, R., Acton, T.B., Montelione, G.T., Hunt, J.F., Tong, L., Northeast Structural Genomics Consortium (NESG)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.69 Å
R-Value Free: 0.228
R-Value Work: 0.185
R-Value Observed: 0.188

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Crystal structure of the yslB protein from Bacillus subtilis.

Vorobiev, S., Abashidze, M., Seetharaman, J., Wang, D., Cunningham, K., Ma, L.-C., Owens, L., Fang, Y., Xiao, R., Acton, T.B., Montelione, G.T., Hunt, J.F., Tong, L.

To be published.

Macromolecule Content

Total Structure Weight: 37.85 kDa
Atom Count: 2,631
Modelled Residue Count: 299
Deposited Residue Count: 316
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
YSLB protein	A, B	158	Bacillus subtilis subsp. subtilis str. 168	Mutation(s): 0 Gene Names: BSU28460, yslB
UniProt
Find proteins for P42955 (Bacillus subtilis (strain 168)) Explore P42955 Go to UniProtKB: P42955
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P42955
Sequence Annotations Expand
Reference Sequence

Small Molecules

Modified Residues 1 Unique
ID	Chains	Type	Formula	2D Diagram	Parent
MSE Query on MSE	A, B	L-PEPTIDE LINKING	C₅ H₁₁ N O₂ Se		MET

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.69 Å
R-Value Free: 0.228
R-Value Work: 0.185
R-Value Observed: 0.188
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 49.827	α = 90
b = 52.105	β = 90
c = 136.868	γ = 90

Software Package:

Software Name	Purpose
SHELXDE	phasing
PHENIX	refinement
HKL-2000	data reduction
SCALEPACK	data scaling

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2010-09-22

Deposition Author(s):

Seetharaman, J.

Montelione, G.T.

Northeast Structural Genomics Consortium (NESG)

Revision History (Full details and data files)

Version 1.0: 2010-09-22
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance
Version 1.2: 2017-10-25
Changes: Author supporting evidence
Version 1.3: 2018-01-24
Changes: Structure summary

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.