3N8E

Substrate binding domain of the human Heat Shock 70kDa protein 9 (mortalin)

PDB DOI: https://doi.org/10.2210/pdb3N8E/pdb

Classification: CHAPERONE
Organism(s): Homo sapiens
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2010-05-28 Released: 2010-06-16
Deposition Author(s): Wisniewska, M., Karlberg, T., Arrowsmith, C.H., Berglund, H., Bountra, C., Collins, R., Edwards, A.M., Flodin, S., Flores, A., Graslund, S., Hammarstrom, M., Johansson, I., Kotenyova, T., Moche, M., Nordlund, P., Nyman, T., Persson, C., Schutz, P., Siponen, M.I., Svensson, L., Thorsell, A.G., Tresaugues, L., van der Berg, S., Wahlberg, E., Weigelt, J., Welin, M., Schuler, H., Structural Genomics Consortium (SGC)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.80 Å
R-Value Free: 0.242
R-Value Work: 0.206
R-Value Observed: 0.208

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Substrate binding domain of the human Heat Shock 70kDa protein 9 (mortalin)

Wisniewska, M., Karlberg, T., Arrowsmith, C.H., Berglund, H., Bountra, C., Collins, R., Edwards, A.M., Flodin, S., Flores, A., Graslund, S., Hammarstrom, M., Johansson, I., Kotenyova, T., Moche, M., Nordlund, P., Nyman, T., Persson, C., Schutz, P., Siponem, M.I., Svensson, L., Thorsell, A.G., Tresaugues, L., van der Berg, S., Wahlberg, E., Weigelt, J., Welin, M., Schuler, H.

To be published.

Macromolecule Content

Total Structure Weight: 40.07 kDa
Atom Count: 2,473
Modelled Residue Count: 318
Deposited Residue Count: 364
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Stress-70 protein, mitochondrial	A, B	182	Homo sapiens	Mutation(s): 0 Gene Names: GRP75, HSPA9, HSPA9B
UniProt & NIH Common Fund Data Resources
Find proteins for P38646 (Homo sapiens) Explore P38646 Go to UniProtKB: P38646
PHAROS: P38646 GTEx: ENSG00000113013
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P38646
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.80 Å
R-Value Free: 0.242
R-Value Work: 0.206
R-Value Observed: 0.208
Space Group: P 6₂ 2 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 118.68	α = 90
b = 118.68	β = 90
c = 159.87	γ = 120

Software Package:

Software Name	Purpose
HKL-2000	data collection
MOLREP	phasing
BUSTER	refinement
XDS	data reduction
XSCALE	data scaling

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2010-06-16

Deposition Author(s):

Arrowsmith, C.H.

Hammarstrom, M.

van der Berg, S.

Structural Genomics Consortium (SGC)

Revision History (Full details and data files)

Version 1.0: 2010-06-16
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance
Version 1.2: 2018-01-24
Changes: Database references
Version 1.3: 2024-02-21
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.