3N35
Erythrina corallodendron lectin mutant (Y106G) with N-Acetylgalactosamine
- PDB DOI: https://doi.org/10.2210/pdb3N35/pdb
- Classification: SUGAR BINDING PROTEIN
- Organism(s): Erythrina corallodendron
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2010-05-19 Released: 2011-03-30
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.189
- R-Value Work: 0.177
- R-Value Observed: 0.177
This is version 2.1 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Lectin | 242 | Erythrina corallodendron | Mutation(s): 1 |  | |
UniProt | |||||
Find proteins for P16404 (Erythrina corallodendron) Explore P16404 Go to UniProtKB: P16404 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P16404 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| A2G Query on A2G | D [auth A] | 2-acetamido-2-deoxy-alpha-D-galactopyranose C8 H15 N O6 OVRNDRQMDRJTHS-CBQIKETKSA-N |  | ||
| MN Query on MN | B [auth A] | MANGANESE (II) ION Mn WAEMQWOKJMHJLA-UHFFFAOYSA-N |  | ||
| CA Query on CA | C [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.189
- R-Value Work: 0.177
- R-Value Observed: 0.177
- Space Group: P 31 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 102.514 | α = 90 |
| b = 102.514 | β = 90 |
| c = 57.023 | γ = 120 |
| Software Name | Purpose |
|---|---|
| MAR345dtb | data collection |
| PHASER | phasing |
| CNS | refinement |
| DENZO | data reduction |
| SCALEPACK | data scaling |
Entry History
Deposition Data
- Released Date: 2011-03-30 Deposition Author(s): Thamotharan, S., Karthikeyan, T., Kulkarni, K.A., Shetty, K.N., Surolia, A., Vijayan, M., Suguna, K.
Revision History (Full details and data files)
- Version 1.0: 2011-03-30
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Database references, Derived calculations, Structure summary - Version 2.1: 2023-11-01
Changes: Data collection, Database references, Refinement description, Structure summary
