Download MendeleyCrystal Structure of Nh3-Dependent Nad+ Synthetase from Streptomyces Avermitilis
Patskovsky, Y., Toro, R., Freeman, J., Do, J., Sauder, J.M., Gerlt, J.A., Burley, S.K., Almo, S.C.To be published.
3N05
CRYSTAL STRUCTURE OF NH3-DEPENDENT NAD+ SYNTHETASE FROM STREPTOMYCES AVERMITILIS
- PDB DOI: https://doi.org/10.2210/pdb3N05/pdb
- Classification: LIGASE
- Organism(s): Streptomyces avermitilis
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2010-05-13 Released: 2010-07-07
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.35 Å
- R-Value Free: 0.268
- R-Value Work: 0.232
- R-Value Observed: 0.233
wwPDB Validation 3D Report Full Report
This is version 1.4 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| NH(3)-dependent NAD(+) synthetase | 590 | Streptomyces avermitilis | Mutation(s): 0 Gene Names: nadE, SAV5948, SAV_5948 |  | |
UniProt | |||||
Find proteins for Q82AV7 (Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)) Explore Q82AV7 Go to UniProtKB: Q82AV7 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q82AV7 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SO4 Query on SO4 | C [auth A] D [auth A] E [auth A] F [auth B] G [auth B] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.35 Å
- R-Value Free: 0.268
- R-Value Work: 0.232
- R-Value Observed: 0.233
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 100.628 | α = 90 |
| b = 108.771 | β = 90 |
| c = 126.643 | γ = 90 |
| Software Name | Purpose |
|---|---|
| SHELX | model building |
| RESOLVE | model building |
| REFMAC | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
| SHELX | phasing |
| RESOLVE | phasing |
Entry History
Deposition Data
- Released Date: 2010-07-07 Deposition Author(s): Patskovsky, Y., Toro, R., Freeman, J., Do, J., Sauder, J.M., Almo, S.C., Burley, S.K., New York SGX Research Center for Structural Genomics (NYSGXRC)
Revision History (Full details and data files)
- Version 1.0: 2010-07-07
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2018-11-21
Changes: Data collection, Structure summary - Version 1.3: 2021-02-10
Changes: Database references, Derived calculations, Structure summary - Version 1.4: 2024-02-21
Changes: Data collection, Database references
