3MVP

The Crystal Structure of a TetR/AcrR transcriptional regulator from Streptococcus mutans to 1.85A

PDB DOI: https://doi.org/10.2210/pdb3MVP/pdb

Classification: transcription regulator
Organism(s): Streptococcus mutans UA159
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2010-05-04 Released: 2010-05-12
Deposition Author(s): Stein, A.J., Xu, X., Cui, H., Chin, S., Savchenko, A., Joachimiak, A., Midwest Center for Structural Genomics (MCSG)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.85 Å
R-Value Free: 0.259
R-Value Work: 0.221
R-Value Observed: 0.223

wwPDB Validation 3D Report Full Report

This is version 1.2 of the entry. See complete history.

Literature

The Crystal Structure of a TetR/AcrR transcriptional regulator from Streptococcus mutans to 1.85A

Stein, A.J., Xu, X., Cui, H., Chin, S., Savchenko, A., Joachimiak, A.

To be published.

Macromolecule Content

Total Structure Weight: 50.83 kDa
Atom Count: 3,381
Modelled Residue Count: 398
Deposited Residue Count: 434
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
TetR/AcrR transcriptional regulator	A, B	217	Streptococcus mutans UA159	Mutation(s): 0 Gene Names: SMU_134
UniProt
Find proteins for Q8DWD0 (Streptococcus mutans serotype c (strain ATCC 700610 / UA159)) Explore Q8DWD0 Go to UniProtKB: Q8DWD0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q8DWD0
Sequence Annotations Expand
Reference Sequence

Small Molecules

Modified Residues 1 Unique
ID	Chains	Type	Formula	2D Diagram	Parent
MSE Query on MSE	A, B	L-PEPTIDE LINKING	C₅ H₁₁ N O₂ Se		MET

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.85 Å
R-Value Free: 0.259
R-Value Work: 0.221
R-Value Observed: 0.223
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 50.49	α = 90
b = 80.197	β = 90
c = 110.63	γ = 90

Software Package:

Software Name	Purpose
DENZO	data reduction
SCALEPACK	data scaling
REFMAC	refinement
PDB_EXTRACT	data extraction
SBC-Collect	data collection
HKL-3000	data reduction
HKL-3000	data scaling
SHELX	phasing
MLPHARE	phasing
DM	phasing
ARP/wARP	model building
Coot	model building

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2010-05-12

Deposition Author(s):

Midwest Center for Structural Genomics (MCSG)

Revision History (Full details and data files)

Version 1.0: 2010-05-12
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance
Version 1.2: 2017-11-08
Changes: Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.