Download MendeleyThe Crystal Structure of a Homoserine Dehydrogenase from Thiobacillus denitrificans to 2.15A
Stein, A.J., Cui, H., Chin, S., Savchenko, A., Joachimiak, A.To be published.
3MTJ
The Crystal Structure of a Homoserine Dehydrogenase from Thiobacillus denitrificans to 2.15A
- PDB DOI: https://doi.org/10.2210/pdb3MTJ/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Thiobacillus denitrificans ATCC 25259
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2010-04-30 Released: 2010-05-12
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.15 Å
- R-Value Free: 0.256
- R-Value Work: 0.217
- R-Value Observed: 0.219
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Homoserine dehydrogenase | 444 | Thiobacillus denitrificans ATCC 25259 | Mutation(s): 0 Gene Names: Tbd_0843 EC: 1.1.1.3 |  | |
UniProt | |||||
Find proteins for Q3SKI5 (Thiobacillus denitrificans (strain ATCC 25259)) Explore Q3SKI5 Go to UniProtKB: Q3SKI5 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q3SKI5 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SO4 Query on SO4 | B [auth A], C [auth A], D [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.15 Å
- R-Value Free: 0.256
- R-Value Work: 0.217
- R-Value Observed: 0.219
- Space Group: P 42 21 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 67.613 | α = 90 |
| b = 67.613 | β = 90 |
| c = 224.279 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| SBC-Collect | data collection |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
| SHELX | phasing |
| MLPHARE | phasing |
| DM | phasing |
| ARP/wARP | model building |
| Coot | model building |
Entry History
Deposition Data
- Released Date: 2010-05-12 Deposition Author(s): Stein, A.J., Cui, H., Chin, S., Savchenko, A., Joachimiak, A., Midwest Center for Structural Genomics (MCSG)
Revision History (Full details and data files)
- Version 1.0: 2010-05-12
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2017-11-08
Changes: Refinement description
