Download MendeleyThe Crystal Structure of a rRNA Methylase from Streptococcus thermophilus to 1.95A
Stein, A.J., Mulligan, R., Freeman, L., Joachimiak, A.To be published.
3MTI
The Crystal Structure of a rRNA Methylase from Streptococcus thermophilus to 1.95A
- PDB DOI: https://doi.org/10.2210/pdb3MTI/pdb
- Classification: TRANSFERASE
- Organism(s): Streptococcus thermophilus LMG 18311
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2010-04-30 Released: 2010-05-12
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.95 Å
- R-Value Free: 0.272
- R-Value Work: 0.238
- R-Value Observed: 0.240
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| rRNA methylase | 185 | Streptococcus thermophilus LMG 18311 | Mutation(s): 0 Gene Names: stu1645 |  | |
UniProt | |||||
Find proteins for Q5M2Z6 (Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311)) Explore Q5M2Z6 Go to UniProtKB: Q5M2Z6 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q5M2Z6 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A, B | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.95 Å
- R-Value Free: 0.272
- R-Value Work: 0.238
- R-Value Observed: 0.240
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 48.331 | α = 90 |
| b = 57.044 | β = 109.11 |
| c = 75.365 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| SBC-Collect | data collection |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
| SHELX | phasing |
| MLPHARE | phasing |
| DM | phasing |
| ARP/wARP | model building |
| Coot | model building |
Entry History
Deposition Data
- Released Date: 2010-05-12 Deposition Author(s): Stein, A.J., Mulligan, R., Freeman, L., Joachimiak, A., Midwest Center for Structural Genomics (MCSG)
Revision History (Full details and data files)
- Version 1.0: 2010-05-12
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2017-11-08
Changes: Refinement description
