Download MendeleyCrystal structure of putative dna cytosine methylase from shigella flexneri 2a str. 2457T
Ramagopal, U.A., Malashkevich, V.N., Toro, R., Sauder, J.M., Burley, S.K., Almo, S.C.To be published.
3ME5
Crystal structure of putative dna cytosine methylase from shigella flexneri 2a str. 2457T
- PDB DOI: https://doi.org/10.2210/pdb3ME5/pdb
- Classification: TRANSFERASE
- Organism(s): Shigella flexneri 2a str. 2457T
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2010-03-31 Released: 2010-04-21
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.75 Å
- R-Value Free: 0.261
- R-Value Work: 0.220
- R-Value Observed: 0.222
wwPDB Validation 3D Report Full Report
This is version 1.5 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Cytosine-specific methyltransferase | 482 | Shigella flexneri 2a str. 2457T | Mutation(s): 0 Gene Names: AAP17376.1, dcm, S2100, SF2005, SF2457T_1495 EC: 2.1.1.37 |  | |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.75 Å
- R-Value Free: 0.261
- R-Value Work: 0.220
- R-Value Observed: 0.222
- Space Group: P 21 21 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 149.629 | α = 90 |
| b = 51.593 | β = 90 |
| c = 63.026 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| CBASS | data collection |
| HKL-2000 | data reduction |
Entry History
Deposition Data
- Released Date: 2010-04-21 Deposition Author(s): Ramagopal, U.A., Malashkevich, V.N., Toro, R., Sauder, J.M., Burley, S.K., Almo, S.C., New York SGX Research Center for Structural Genomics (NYSGXRC)
Revision History (Full details and data files)
- Version 1.0: 2010-04-21
Type: Initial release - Version 1.1: 2011-07-13
Changes: Advisory, Refinement description, Version format compliance - Version 1.2: 2012-05-23
Changes: Database references, Source and taxonomy - Version 1.3: 2017-11-08
Changes: Refinement description - Version 1.4: 2018-11-21
Changes: Data collection, Structure summary - Version 1.5: 2021-02-10
Changes: Database references, Derived calculations, Structure summary
