Download MendeleyStructure of stringent starvation protein A (sspA) from Pseudomonas putida
Ramagopal, U.A., Toro, R., Burley, S.K., Almo, S.C.To be published.
3MDK
Structure of stringent starvation protein A (sspA) from Pseudomonas putida
- PDB DOI: https://doi.org/10.2210/pdb3MDK/pdb
- Classification: transcriptional regulator
- Organism(s): Pseudomonas putida KT2440
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2010-03-30 Released: 2010-05-12
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.258
- R-Value Work: 0.216
- R-Value Observed: 0.218
wwPDB Validation 3D Report Full Report
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Stringent starvation protein A | 217 | Pseudomonas putida KT2440 | Mutation(s): 0 Gene Names: PP1320, sspA |  | |
UniProt | |||||
Find proteins for Q88N92 (Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)) Explore Q88N92 Go to UniProtKB: Q88N92 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q88N92 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A, B | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.258
- R-Value Work: 0.216
- R-Value Observed: 0.218
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 108.06 | α = 90 |
| b = 48.916 | β = 107.24 |
| c = 91.388 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| CBASS | data collection |
| HKL-2000 | data reduction |
| SHELXE | model building |
| SHELXD | phasing |
| CCP4 | phasing |
Entry History
Deposition Data
- Released Date: 2010-05-12 Deposition Author(s): Ramagopal, U.A., Toro, R., Burley, S.K., Almo, S.C., New York SGX Research Center for Structural Genomics (NYSGXRC)
Revision History (Full details and data files)
- Version 1.0: 2010-05-12
Type: Initial release - Version 1.1: 2011-07-13
Changes: Advisory, Refinement description, Version format compliance - Version 1.2: 2017-11-08
Changes: Refinement description - Version 1.3: 2021-02-10
Changes: Database references, Derived calculations, Structure summary
