Download MendeleyThe Crystal Structure of a Secreted Thiol-disulfide Isomerase from Corynebacterium glutamicum to 1.75A
Stein, A.J., Weger, A., Hendricks, R., Cobb, G., Joachimiak, A.To be published.
3LWA
The Crystal Structure of a Secreted Thiol-disulfide Isomerase from Corynebacterium glutamicum to 1.75A
- PDB DOI: https://doi.org/10.2210/pdb3LWA/pdb
- Classification: ISOMERASE
- Organism(s): Corynebacterium glutamicum
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2010-02-23 Released: 2010-03-02
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.75 Å
- R-Value Free: 0.230
- R-Value Work: 0.200
- R-Value Observed: 0.201
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Secreted thiol-disulfide isomerase | 183 | Corynebacterium glutamicum | Mutation(s): 0 Gene Names: cg0520, Cgl0439 |  | |
UniProt | |||||
Find proteins for Q8NT71 (Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)) Explore Q8NT71 Go to UniProtKB: Q8NT71 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q8NT71 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| CA Query on CA | B [auth A], C [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.75 Å
- R-Value Free: 0.230
- R-Value Work: 0.200
- R-Value Observed: 0.201
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 38.818 | α = 90 |
| b = 35.376 | β = 96.69 |
| c = 53.718 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| SBC-Collect | data collection |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
| SHELX | phasing |
| MLPHARE | phasing |
| RESOLVE | phasing |
| ARP/wARP | model building |
| Coot | model building |
Entry History
Deposition Data
- Released Date: 2010-03-02 Deposition Author(s): Stein, A.J., Weger, A., Hendricks, R., Cobb, G., Joachimiak, A., Midwest Center for Structural Genomics (MCSG)
Revision History (Full details and data files)
- Version 1.0: 2010-03-02
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2017-11-08
Changes: Refinement description
