3LAY

Alpha-Helical barrel formed by the decamer of the zinc resistance-associated protein (STM4172) from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

PDB DOI: https://doi.org/10.2210/pdb3LAY/pdb

Classification: METAL BINDING PROTEIN
Organism(s): Salmonella enterica subsp. enterica serovar Typhimurium
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2010-01-07 Released: 2010-01-19
Deposition Author(s): Filippova, E.V., Minasov, G., Shuvalova, L., Winsor, J., Dubrovska, I., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.70 Å
R-Value Free: 0.267
R-Value Work: 0.223
R-Value Observed: 0.225

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Alpha-Helical barrel formed by the decamer of the zinc resistance-associated protein (STM4172) from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Filippova, E.V., Minasov, G., Shuvalova, L., Winsor, J., Dubrovska, I., Papazisi, L., Anderson, W.F.

To be published.

Macromolecule Content

Total Structure Weight: 188.92 kDa
Atom Count: 6,014
Modelled Residue Count: 787
Deposited Residue Count: 1,750
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Zinc resistance-associated protein	A, B, C, D, E A, B, C, D, E, F, G, H, I, J	175	Salmonella enterica subsp. enterica serovar Typhimurium	Mutation(s): 0 Gene Names: STM4172, STMF1.61, zraP
UniProt
Find proteins for Q9L9I0 (Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)) Explore Q9L9I0 Go to UniProtKB: Q9L9I0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q9L9I0
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.70 Å
R-Value Free: 0.267
R-Value Work: 0.223
R-Value Observed: 0.225
Space Group: P 2₁ 2₁ 2
Diffraction Data: https://doi.org/10.18430/M33LAY

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 100.795	α = 90
b = 148.91	β = 90
c = 74.536	γ = 90

Software Package:

Software Name	Purpose
Blu-Ice	data collection
MLPHARE	phasing
DM	model building
SHELXD	phasing
RESOLVE	model building
CCP4	model building
ARP/wARP	model building
Coot	model building
REFMAC	refinement
HKL-2000	data reduction
HKL-3000	data scaling
DM	phasing
RESOLVE	phasing
CCP4	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2010-01-19

Deposition Author(s):

Filippova, E.V.

Center for Structural Genomics of Infectious Diseases (CSGID)

Revision History (Full details and data files)

Version 1.0: 2010-01-19
Type: Initial release
Version 1.1: 2011-07-13
Changes: Advisory, Version format compliance
Version 1.2: 2017-11-01
Changes: Refinement description
Version 1.3: 2024-02-21
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.