Download MendeleyCrystal structure of a LacI family transcriptional regulator from Mycobacterium smegmatis
Bonanno, J.B., Rutter, M., Bain, K.T., Do, J., Ozyurt, S., Wasserman, S., Sauder, J.M., Burley, S.K., Almo, S.C.To be published.
3KKE
Crystal structure of a LacI family transcriptional regulator from Mycobacterium smegmatis
- PDB DOI: https://doi.org/10.2210/pdb3KKE/pdb
- Classification: transcription regulator
- Organism(s): Mycolicibacterium smegmatis MC2 155
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2009-11-05 Released: 2009-12-08
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.247
- R-Value Work: 0.194
- R-Value Observed: 0.197
wwPDB Validation 3D Report Full Report
This is version 1.5 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| LacI family Transcriptional regulator | 303 | Mycolicibacterium smegmatis MC2 155 | Mutation(s): 0 Gene Names: MSMEG_0491 |  | |
UniProt | |||||
Find proteins for A0QPR6 (Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)) Explore A0QPR6 Go to UniProtKB: A0QPR6 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0QPR6 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ACT Query on ACT | E [auth A], F [auth A], G [auth B], H [auth C], I [auth D] | ACETATE ION C2 H3 O2 QTBSBXVTEAMEQO-UHFFFAOYSA-M |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.247
- R-Value Work: 0.194
- R-Value Observed: 0.197
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 64.599 | α = 90 |
| b = 65.468 | β = 90.72 |
| c = 116.034 | γ = 90 |
| Software Name | Purpose |
|---|---|
| SCALA | data scaling |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| MAR345 | data collection |
| MOSFLM | data reduction |
| SHELXCD | phasing |
| SHELXE | model building |
| MLPHARE | phasing |
| DM | phasing |
Entry History
Deposition Data
- Released Date: 2009-12-08 Deposition Author(s): Bonanno, J.B., Rutter, M., Bain, K.T., Do, J., Ozyurt, S., Wasserman, S., Sauder, J.M., Burley, S.K., Almo, S.C., New York SGX Research Center for Structural Genomics (NYSGXRC)
Revision History (Full details and data files)
- Version 1.0: 2009-12-08
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2017-11-01
Changes: Refinement description - Version 1.3: 2018-11-21
Changes: Data collection, Structure summary - Version 1.4: 2021-02-10
Changes: Database references, Derived calculations, Structure summary - Version 1.5: 2024-02-21
Changes: Data collection, Database references
