Download MendeleyX-ray Crystal Structure of the Human Replication Protein A Complex from Wheat Germ Cell Free Expression
Makino, S.-I., Burgie, E.S., Bingman, C.A., Fox, B.G., Phillips Jr., G.N.To be published.
3KDF
X-ray Crystal Structure of the Human Replication Protein A Complex from Wheat Germ Cell Free Expression
- PDB DOI: https://doi.org/10.2210/pdb3KDF/pdb
- Classification: REPLICATION
- Organism(s): Homo sapiens
- Mutation(s): Yes
- Deposited: 2009-10-22 Released: 2009-12-01
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.98 Å
- R-Value Free: 0.206
- R-Value Work: 0.192
- R-Value Observed: 0.192
wwPDB Validation 3D Report Full Report
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Replication protein A 14 kDa subunit | 121 | Homo sapiens | Mutation(s): 1 Gene Names: REPA3, RPA14, RPA3 |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P35244 (Homo sapiens) Explore P35244 Go to UniProtKB: P35244 | |||||
PHAROS: P35244 GTEx: ENSG00000106399 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P35244 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Replication protein A 32 kDa subunit | B [auth D], D [auth B] | 132 | Homo sapiens | Mutation(s): 1 Gene Names: REPA2, RPA2, RPA32, RPA34 |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P15927 (Homo sapiens) Explore P15927 Go to UniProtKB: P15927 | |||||
PHAROS: P15927 GTEx: ENSG00000117748 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P15927 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| EDO Query on EDO | E [auth A] F [auth A] G [auth D] H [auth D] I [auth C] | 1,2-ETHANEDIOL C2 H6 O2 LYCAIKOWRPUZTN-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A, C | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.98 Å
- R-Value Free: 0.206
- R-Value Work: 0.192
- R-Value Observed: 0.192
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 66.68 | α = 90 |
| b = 77.7 | β = 90 |
| c = 121.3 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| SHARP | phasing |
| DM | phasing |
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
| HKL-2000 | data collection |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
Entry History
Deposition Data
- Released Date: 2009-12-01 Deposition Author(s): Burgie, E.S., Bingman, C.A., Phillips Jr., G.N., Fox, B.G., Makino, S.-I., Center for Eukaryotic Structural Genomics (CESG)
Revision History (Full details and data files)
- Version 1.0: 2009-12-01
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2017-11-01
Changes: Refinement description - Version 1.3: 2021-10-13
Changes: Database references, Derived calculations
