3IXR

Crystal Structure of Xylella fastidiosa PrxQ C47S Mutant

PDB DOI: https://doi.org/10.2210/pdb3IXR/pdb

Classification: OXIDOREDUCTASE
Organism(s): Xylella fastidiosa
Expression System: Escherichia coli BL21(DE3)
Mutation(s): Yes

Deposited: 2009-09-04 Released: 2010-03-23
Deposition Author(s): Horta, B.B., Oliveira, M.A., Discola, K.F., Cussiol, J.R.R., Netto, L.E.S.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.60 Å
R-Value Free: 0.247
R-Value Work: 0.196
R-Value Observed: 0.198

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Biochemical and Structural Analysis Indicates that PrxQ, a Cys Based Peroxidase from Xylella fastidiosa, possesses high reactivity towards hydroperoxides

Horta, B.B., Oliveira, M.A., Discola, K.F., Cussiol, J.R.R., Netto, L.E.S.

To be published.

Macromolecule Content

Total Structure Weight: 19.99 kDa
Atom Count: 1,415
Modelled Residue Count: 159
Deposited Residue Count: 179
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Bacterioferritin comigratory protein	A	179	Xylella fastidiosa	Mutation(s): 1 Gene Names: Peroxiredoxin Q, XF_0961 EC: 1.11.1.15
UniProt
Find proteins for Q9PER7 (Xylella fastidiosa (strain 9a5c)) Explore Q9PER7 Go to UniProtKB: Q9PER7
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q9PER7
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.60 Å
R-Value Free: 0.247
R-Value Work: 0.196
R-Value Observed: 0.198
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 38.973	α = 90
b = 39.743	β = 90
c = 99.836	γ = 90

Software Package:

Software Name	Purpose
MAR345dtb	data collection
PHASER	phasing
REFMAC	refinement
MOSFLM	data reduction
SCALA	data scaling

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2010-03-23

Deposition Author(s):

Cussiol, J.R.R.

Revision History (Full details and data files)

Version 1.0: 2010-03-23
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance
Version 1.2: 2021-10-13
Changes: Database references
Version 1.3: 2023-09-06
Changes: Data collection, Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.