Download MendeleyCRYSTAL STRUCTURE OF GALACTOSE 1-EPIMERASE FROM Lactobacillus acidophilus
Patskovsky, Y., Toro, R., Dickey, M., Chang, S., Sauder, J.M., Burley, S.K., Almo, S.C.To be published.
3IMH
CRYSTAL STRUCTURE OF GALACTOSE 1-EPIMERASE FROM Lactobacillus acidophilus NCFM
- PDB DOI: https://doi.org/10.2210/pdb3IMH/pdb
- Classification: ISOMERASE
- Organism(s): Lactobacillus acidophilus NCFM
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2009-08-10 Released: 2009-08-18
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.76 Å
- R-Value Free: 0.170
- R-Value Work: 0.140
- R-Value Observed: 0.141
wwPDB Validation 3D Report Full Report
This is version 1.6 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Galactose-1-epimerase | 338 | Lactobacillus acidophilus NCFM | Mutation(s): 1 Gene Names: galM, LBA1457 EC: 5.1.3.3 |  | |
UniProt | |||||
Find proteins for Q5FJ46 (Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)) Explore Q5FJ46 Go to UniProtKB: Q5FJ46 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q5FJ46 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| GOL Query on GOL | F [auth A] G [auth A] H [auth A] I [auth A] K [auth A] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
| CL Query on CL | E [auth A], J [auth A], P [auth B] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
| MG Query on MG | C [auth A], D [auth A], L [auth B] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.76 Å
- R-Value Free: 0.170
- R-Value Work: 0.140
- R-Value Observed: 0.141
- Space Group: P 4 21 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 134.683 | α = 90 |
| b = 134.683 | β = 90 |
| c = 103.027 | γ = 90 |
| Software Name | Purpose |
|---|---|
| SHELXCD | phasing |
| SHELXD | phasing |
| SHELXE | model building |
| REFMAC | refinement |
| DENZO | data reduction |
| HKL-2000 | data scaling |
Entry History
Deposition Data
- Released Date: 2009-08-18 Deposition Author(s): Patskovsky, Y., Toro, R., Dickey, M., Chang, S., Sauder, J.M., Burley, S.K., Almo, S.C., New York SGX Research Center for Structural Genomics (NYSGXRC)
Revision History (Full details and data files)
- Version 1.0: 2009-08-18
Type: Initial release - Version 1.1: 2011-07-13
Changes: Source and taxonomy, Version format compliance - Version 1.2: 2017-11-01
Changes: Refinement description - Version 1.3: 2018-11-21
Changes: Data collection, Structure summary - Version 1.4: 2021-02-10
Changes: Database references, Derived calculations, Structure summary - Version 1.5: 2021-10-13
Changes: Database references - Version 1.6: 2024-02-21
Changes: Data collection
