3IEC

Helicobacter pylori CagA Inhibits PAR1/MARK Family Kinases by Mimicking Host Substrates


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.20 Å
  • R-Value Free: 0.248 
  • R-Value Work: 0.204 
  • R-Value Observed: 0.206 

wwPDB Validation   3D Report Full Report


This is version 1.4 of the entry. See complete history


Literature

Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates.

Nesic, D.Miller, M.C.Quinkert, Z.T.Stein, M.Chait, B.T.Stebbins, C.E.

(2010) Nat Struct Mol Biol 17: 130-132

  • DOI: https://doi.org/10.1038/nsmb.1705
  • Primary Citation of Related Structures:  
    3IEC

  • PubMed Abstract: 

    The CagA protein of Helicobacter pylori interacts with numerous cellular factors and is associated with increased virulence and risk of gastric carcinoma. We present here the cocrystal structure of a subdomain of CagA with the human kinase PAR1b/MARK2, revealing that a CagA peptide mimics substrates of this kinase family, resembling eukaryotic protein kinase inhibitors. Mutagenesis of conserved residues central to this interaction renders CagA inactive as an inhibitor of MARK2.


  • Organizational Affiliation

    Laboratory of Structural Microbiology, Rockefeller University, New York, New York, USA.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Serine/threonine-protein kinase MARK2
A, B, C, D
319Homo sapiensMutation(s): 1 
Gene Names: CagAEMK1MARK2
EC: 2.7.11.1
UniProt & NIH Common Fund Data Resources
Find proteins for Q7KZI7 (Homo sapiens)
Explore Q7KZI7 
Go to UniProtKB:  Q7KZI7
PHAROS:  Q7KZI7
GTEx:  ENSG00000072518 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ7KZI7
Sequence Annotations
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  • Reference Sequence
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
Cytotoxicity-associated immunodominant antigen
E, F, G, H
125Helicobacter pyloriMutation(s): 0 
Gene Names: cagAcai
UniProt
Find proteins for P55980 (Helicobacter pylori (strain ATCC 700392 / 26695))
Explore P55980 
Go to UniProtKB:  P55980
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP55980
Sequence Annotations
Expand
  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.20 Å
  • R-Value Free: 0.248 
  • R-Value Work: 0.204 
  • R-Value Observed: 0.206 
  • Space Group: P 1 21 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 93.466α = 90
b = 93.256β = 100.94
c = 113.955γ = 90
Software Package:
Software NamePurpose
DENZOdata reduction
SCALEPACKdata scaling
PHASERphasing
REFMACrefinement
PDB_EXTRACTdata extraction
HKL-2000data collection

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2009-12-08
    Type: Initial release
  • Version 1.1: 2011-07-13
    Changes: Advisory, Version format compliance
  • Version 1.2: 2017-11-01
    Changes: Refinement description
  • Version 1.3: 2021-10-13
    Changes: Database references
  • Version 1.4: 2024-02-21
    Changes: Data collection