Download MendeleyMechanisms of allosteric activation of the DegS protease by OMP-peptide binding and protein-substrate binding
Sohn, J., Grant, R.A., Sauer, R.T.To be published.
3GCN
Crystal structure of DegS H198P/D320A mutant modified by DFP in complex with OMP peptide (YQF)
- PDB DOI: https://doi.org/10.2210/pdb3GCN/pdb
- Classification: HYDROLASE/HYDROLASE ACTIVATOR
- Organism(s): Escherichia coli K-12
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2009-02-22 Released: 2009-03-31
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 3.00 Å
- R-Value Free: 0.249
- R-Value Work: 0.213
- R-Value Observed: 0.215
wwPDB Validation 3D Report Full Report
This is version 1.5 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Protease degS | 340 | Escherichia coli K-12 | Mutation(s): 2 Gene Names: b3235, degS, hhoB, htrH, JW3204 EC: 3.4.21 |  | |
UniProt | |||||
Find proteins for P0AEE3 (Escherichia coli (strain K12)) Explore P0AEE3 Go to UniProtKB: P0AEE3 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0AEE3 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by: Sequence | 3D Structure
Small Molecules
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MIS Query on MIS | A | L-PEPTIDE LINKING | C6 H14 N O6 P |  | SER |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 3.00 Å
- R-Value Free: 0.249
- R-Value Work: 0.213
- R-Value Observed: 0.215
- Space Group: P 21 3
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 119.03 | α = 90 |
| b = 119.03 | β = 90 |
| c = 119.03 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| PHASER | phasing |
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
| HKL-2000 | data collection |
Entry History
Deposition Data
- Released Date: 2009-03-31 Deposition Author(s): Sohn, J., Sauer, R.T., Grant, R.A.
Revision History (Full details and data files)
- Version 1.0: 2009-03-31
Type: Initial release - Version 1.1: 2011-07-13
Changes: Source and taxonomy, Version format compliance - Version 1.2: 2012-12-12
Changes: Other - Version 1.3: 2013-03-13
Changes: Other - Version 1.4: 2017-11-01
Changes: Refinement description - Version 1.5: 2021-10-20
Changes: Database references, Derived calculations
