3F5L
Semi-active E176Q mutant of rice BGlu1, a plant exoglucanase/beta-glucosidase
- PDB DOI: https://doi.org/10.2210/pdb3F5L/pdb
- Classification: HYDROLASE
- Organism(s): Oryza sativa Japonica Group
- Expression System: Escherichia coli
- Mutation(s): Yes 
- Deposited: 2008-11-04 Released: 2009-11-03 
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.37 Å
- R-Value Free: 0.198 
- R-Value Work: 0.181 
- R-Value Observed: 0.182 
wwPDB Validation   3D Report Full Report
This is version 2.1 of the entry. See complete history. 
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
Beta-glucosidase | 481 | Oryza sativa Japonica Group | Mutation(s): 1  Gene Names: Os3bglu7 EC: 3.2.1.21 | ||
UniProt | |||||
Find proteins for Q75I93 (Oryza sativa subsp. japonica) Explore Q75I93  Go to UniProtKB:  Q75I93 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | Q75I93 | ||||
Sequence AnnotationsExpand | |||||
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Oligosaccharides
Small Molecules
Ligands 3 Unique | |||||
---|---|---|---|---|---|
ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
MES Query on MES | G [auth A], I [auth B] | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID C6 H13 N O4 S SXGZJKUKBWWHRA-UHFFFAOYSA-N | |||
SO4 Query on SO4 | F [auth A], H [auth B] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L | |||
ZN Query on ZN | E [auth A] | ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N |
Biologically Interesting Molecules (External Reference) 1 Unique
Entity ID: 2 | |||||
---|---|---|---|---|---|
ID | Chains | Name | Type/Class | 2D Diagram | 3D Interactions |
PRD_900024 Query on PRD_900024 | C, D | beta-laminaribiose | Oligosaccharide / Antimicrobial |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.37 Å
- R-Value Free: 0.198 
- R-Value Work: 0.181 
- R-Value Observed: 0.182 
- Space Group: P 21 21 21
Unit Cell:
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 79.624 | α = 90 |
b = 100.804 | β = 90 |
c = 127.631 | γ = 90 |
Software Name | Purpose |
---|---|
HKL-2000 | data collection |
AMoRE | phasing |
REFMAC | refinement |
HKL-2000 | data reduction |
HKL-2000 | data scaling |
Entry History 
Deposition Data
- Released Date: 2009-11-03  Deposition Author(s): Chuenchor, W., Ketudat Cairns, J.R., Pengthaisong, S., Robinson, R.C., Yuvaniyama, J., Chen, C.-J.
Revision History (Full details and data files)
- Version 1.0: 2009-11-03
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Advisory, Atomic model, Data collection, Database references, Derived calculations, Non-polymer description, Structure summary - Version 2.1: 2023-11-01
Changes: Data collection, Database references, Refinement description, Structure summary