3E9E

Structure of full-length H11A mutant form of TIGAR from Danio rerio

PDB DOI: https://doi.org/10.2210/pdb3E9E/pdb

Classification: HYDROLASE
Organism(s): Danio rerio
Expression System: Escherichia coli
Mutation(s): Yes

Deposited: 2008-08-21 Released: 2008-12-16
Deposition Author(s): Li, H., Jogl, G.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.10 Å
R-Value Free: 0.234
R-Value Work: 0.192
R-Value Observed: 0.194

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

TIGAR (TP53 induced glycolysis and apoptosis regulator) is a fructose-2,6- and fructose-1,6-bisphosphatase

Li, H., Jogl, G.

To be published.

Macromolecule Content

Total Structure Weight: 59.28 kDa
Atom Count: 4,083
Modelled Residue Count: 480
Deposited Residue Count: 530
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Zgc:56074	A, B	265	Danio rerio	Mutation(s): 1 Gene Names: zgc:56074
UniProt
Find proteins for Q7ZVE3 (Danio rerio) Explore Q7ZVE3 Go to UniProtKB: Q7ZVE3
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q7ZVE3
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
PO4 Query on PO4 Download Ideal Coordinates CCD File SDF format, chain C [auth A] SDF format, chain D [auth B] MOL2 format, chain C [auth A] MOL2 format, chain D [auth B]	C [auth A], D [auth B]	PHOSPHATE ION O₄ P NBIIXXVUZAFLBC-UHFFFAOYSA-K		Interactions Focus chain C [auth A] Focus chain D [auth B] Interactions & Density Focus chain C [auth A] Focus chain D [auth B]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.10 Å
R-Value Free: 0.234
R-Value Work: 0.192
R-Value Observed: 0.194
Space Group: P 6₅

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 91.358	α = 90
b = 91.358	β = 90
c = 155.875	γ = 120

Software Package:

Software Name	Purpose
DENZO	data reduction
SCALEPACK	data scaling
COMO	phasing
REFMAC	refinement
PDB_EXTRACT	data extraction
HKL-2000	data reduction

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2008-12-16

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2008-12-16
Type: Initial release
Version 1.1: 2011-07-13
Changes: Advisory, Version format compliance
Version 1.2: 2017-10-25
Changes: Refinement description
Version 1.3: 2021-10-20
Changes: Database references, Derived calculations

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.