Download MendeleySite-Selective Glycosylation of Cysteine-93 beta on the Surface of Bovine Hemoglobin and its Application as a Novel Oxygen Therapeutic
Bhatt, V.S., Zhang, Y., Sun, G., Wang, P.G., Palmer, A.F.To be published.
3CIU
Site-Selective Glycosylation of Cysteine-93 beta on the Surface of Bovine Hemoglobin and its Application as a Novel Oxygen Therapeutic
- PDB DOI: https://doi.org/10.2210/pdb3CIU/pdb
- Classification: OXYGEN BINDING
- Organism(s): Bos taurus
- Mutation(s): No
- Deposited: 2008-03-11 Released: 2008-04-15
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 3.50 Å
- R-Value Free: 0.332
- R-Value Work: 0.268
- R-Value Observed: 0.271
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Hemoglobin subunit alpha | 141 | Bos taurus | Mutation(s): 0 Gene Names: HBA |  | |
UniProt | |||||
Find proteins for P01966 (Bos taurus) Explore P01966 Go to UniProtKB: P01966 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P01966 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Hemoglobin subunit beta | 145 | Bos taurus | Mutation(s): 0 Gene Names: HBB |  | |
UniProt | |||||
Find proteins for P02070 (Bos taurus) Explore P02070 Go to UniProtKB: P02070 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P02070 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| 5DP Query on 5DP | I [auth B] | 5-(2,5-dioxopyrrolidin-1-yl)-N-[2-(2-{2-[(4-O-alpha-D-idopyranosyl-alpha-D-mannopyranosyl)oxy]ethoxy}ethoxy)ethyl]pentanamide C27 H46 N2 O16 GIIWJKKPIBKANP-SLHLUNFVSA-N |  | ||
| HEM Query on HEM | E [auth A], G [auth B], J [auth C], L [auth D] | PROTOPORPHYRIN IX CONTAINING FE C34 H32 Fe N4 O4 KABFMIBPWCXCRK-RGGAHWMASA-L |  | ||
| O Query on O | F [auth A], H [auth B], K [auth C], M [auth D] | OXYGEN ATOM O XLYOFNOQVPJJNP-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 3.50 Å
- R-Value Free: 0.332
- R-Value Work: 0.268
- R-Value Observed: 0.271
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 62.604 | α = 90 |
| b = 73.982 | β = 90 |
| c = 129.796 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| CrystalClear | data collection |
| CrystalClear | data reduction |
| CrystalClear | data scaling |
| AMoRE | phasing |
Entry History
Deposition Data
- Released Date: 2008-04-15 Deposition Author(s): Bhatt, V.S., Zhang, Y., Sun, G., Wang, P.G., Palmer, A.F.
Revision History (Full details and data files)
- Version 1.0: 2008-04-15
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2023-08-30
Changes: Data collection, Database references, Derived calculations, Refinement description
