3C65

Crystal Structure of Bacillus stearothermophilus UvrC 5' endonuclease domain

PDB DOI: https://doi.org/10.2210/pdb3C65/pdb

Classification: HYDROLASE
Organism(s): Geobacillus kaustophilus (strain HTA426)
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2008-02-02 Released: 2008-08-05
Deposition Author(s): Pakotiprapha, D., Bowman, B.R., Mueller, A.A., Inuzuka, Y., Verdine, G.L.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.90 Å
R-Value Free: 0.221
R-Value Work: 0.204

wwPDB Validation 3D Report Full Report

This is version 1.2 of the entry. See complete history.

Literature

Crystal Structure of Bacillus stearothermophilus UvrC 5' endonuclease domain

Pakotiprapha, D., Bowman, B.R., Mueller, A.A., Inuzuka, Y., Verdine, G.L.

To be published.

Macromolecule Content

Total Structure Weight: 25.87 kDa
Atom Count: 1,274
Modelled Residue Count: 152
Deposited Residue Count: 226
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
UvrABC system protein C	A	226	N/A	Mutation(s): 0 Gene Names: uvrC
UniProt
Find proteins for Q5KWH6 (Geobacillus kaustophilus (strain HTA426)) Explore Q5KWH6 Go to UniProtKB: Q5KWH6
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q5KWH6
Sequence Annotations Expand
Reference Sequence

Small Molecules

Modified Residues 1 Unique
ID	Chains	Type	Formula	2D Diagram	Parent
MSE Query on MSE	A	L-PEPTIDE LINKING	C₅ H₁₁ N O₂ Se		MET

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.90 Å
R-Value Free: 0.221
R-Value Work: 0.204
Space Group: P 6₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 81.779	α = 90
b = 81.779	β = 90
c = 58.583	γ = 120

Software Package:

Software Name	Purpose
DENZO	data reduction
SCALEPACK	data scaling
SOLOMON	phasing
CNS	refinement
PDB_EXTRACT	data extraction
SHARP	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2008-08-05

Deposition Author(s):

Pakotiprapha, D.

Revision History (Full details and data files)

Version 1.0: 2008-08-05
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance
Version 1.2: 2017-10-25
Changes: Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.