3C3M

Crystal structure of the N-terminal domain of response regulator receiver protein from Methanoculleus marisnigri JR1

PDB DOI: https://doi.org/10.2210/pdb3C3M/pdb

Classification: SIGNALING PROTEIN
Organism(s): Methanoculleus marisnigri JR1
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2008-01-28 Released: 2008-02-05
Deposition Author(s): Patskovsky, Y., Ramagopal, U.A., Toro, R., Meyer, A.J., Dickey, M., Chang, S., Groshong, C., Sauder, J.M., Burley, S.K., Almo, S.C., New York SGX Research Center for Structural Genomics (NYSGXRC)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.70 Å
R-Value Free: 0.193
R-Value Work: 0.164
R-Value Observed: 0.165

wwPDB Validation 3D Report Full Report

This is version 1.4 of the entry. See complete history.

Literature

Crystal structure of the N-terminal domain of response regulator receiver protein from Methanoculleus marisnigri JR1.

Patskovsky, Y., Ramagopal, U.A., Toro, R., Meyer, A.J., Dickey, M., Chang, S., Groshong, C., Sauder, J.M., Burley, S.K., Almo, S.C.

To be published.

Macromolecule Content

Total Structure Weight: 16.23 kDa
Atom Count: 1,230
Modelled Residue Count: 123
Deposited Residue Count: 138
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Response regulator receiver protein	A	138	Methanoculleus marisnigri JR1	Mutation(s): 0 Gene Names: Memar_0295, Memar_0309
UniProt
Find proteins for A3CS79 (Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)) Explore A3CS79 Go to UniProtKB: A3CS79
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A3CS79
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
GOL Query on GOL Download Ideal Coordinates CCD File SDF format, chain B [auth A] SDF format, chain C [auth A] MOL2 format, chain B [auth A] MOL2 format, chain C [auth A]	B [auth A], C [auth A]	GLYCEROL C₃ H₈ O₃ PEDCQBHIVMGVHV-UHFFFAOYSA-N		Interactions Focus chain B [auth A] Focus chain C [auth A] Interactions & Density Focus chain B [auth A] Focus chain C [auth A]

Modified Residues 1 Unique
ID	Chains	Type	Formula	2D Diagram	Parent
MSE Query on MSE	A	L-PEPTIDE LINKING	C₅ H₁₁ N O₂ Se		MET

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.70 Å
R-Value Free: 0.193
R-Value Work: 0.164
R-Value Observed: 0.165
Space Group: P 3₂ 2 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 57.744	α = 90
b = 57.744	β = 90
c = 93.233	γ = 120

Software Package:

Software Name	Purpose
SHELXD	phasing
SHELXE	model building
REFMAC	refinement
ADSC	data collection
HKL-2000	data reduction
SCALEPACK	data scaling

Structure Validation

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Entry History

Deposition Data

Released Date: 2008-02-05

Deposition Author(s):

New York SGX Research Center for Structural Genomics (NYSGXRC)

Revision History (Full details and data files)

Version 1.0: 2008-02-05
Type: Initial release
Version 1.1: 2011-07-13
Changes: Non-polymer description, Version format compliance
Version 1.2: 2017-10-25
Changes: Refinement description
Version 1.3: 2018-11-14
Changes: Data collection, Structure summary
Version 1.4: 2021-02-03
Changes: Database references, Derived calculations, Structure summary

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3C3M

Crystal structure of the N-terminal domain of response regulator receiver protein from Methanoculleus marisnigri JR1

Crystal structure of the N-terminal domain of response regulator receiver protein from Methanoculleus marisnigri JR1.

Entity ID: 1

UniProt

Entity Groups

Sequence Annotations

Expand

Experimental Data

Structure Validation

Deposition Data

Revision History (Full details and data files)