3BX3

Puf4 T650C/C724R Mutant bound to Cox17 RNA 3' UTR recognition sequence


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 3.00 Å
  • R-Value Free: 0.350 
  • R-Value Work: 0.290 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

Basis of altered RNA-binding specificity by PUF proteins revealed by crystal structures of yeast Puf4p

Miller, M.T.Higgin, J.J.Hall, T.M.T.

(2008) Nat Struct Mol Biol 15: 397-402

  • DOI: https://doi.org/10.1038/nsmb.1390
  • Primary Citation of Related Structures:  
    3BWT, 3BX2, 3BX3

  • PubMed Abstract: 

    Pumilio/FBF (PUF) family proteins are found in eukaryotic organisms and regulate gene expression post-transcriptionally by binding to sequences in the 3' untranslated region of target transcripts. PUF proteins contain an RNA binding domain that typically comprises eight alpha-helical repeats, each of which recognizes one RNA base. Some PUF proteins, including yeast Puf4p, have altered RNA binding specificity and use their eight repeats to bind to RNA sequences with nine or ten bases. Here we report the crystal structures of Puf4p alone and in complex with a 9-nucleotide (nt) target RNA sequence, revealing that Puf4p accommodates an 'extra' nucleotide by modest adaptations allowing one base to be turned away from the RNA binding surface. Using structural information and sequence comparisons, we created a mutant Puf4p protein that preferentially binds to an 8-nt target RNA sequence over a 9-nt sequence and restores binding of each protein repeat to one RNA base.


  • Organizational Affiliation

    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T.W. Alexander Drive, Building 101, Room F363, Research Triangle Park, North Carolina 27709, USA.


Macromolecules

Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
Protein PUF4C [auth A],
D [auth B]
335Saccharomyces cerevisiaeMutation(s): 2 
Gene Names: PUF4
UniProt
Find proteins for P25339 (Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Explore P25339 
Go to UniProtKB:  P25339
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP25339
Sequence Annotations
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  • Reference Sequence

Find similar nucleic acids by:  Sequence   |   3D Structure  

Entity ID: 1
MoleculeChains LengthOrganismImage
COX17 RNA target sequenceA [auth C],
B [auth D]
8N/A
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 3.00 Å
  • R-Value Free: 0.350 
  • R-Value Work: 0.290 
  • Space Group: P 21 21 21
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 66.065α = 90
b = 137.116β = 90
c = 160.559γ = 90
Software Package:
Software NamePurpose
HKL-2000data collection
SOLVEphasing
PHENIXrefinement
MOSFLMdata reduction
SCALAdata scaling

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2008-03-11
    Type: Initial release
  • Version 1.1: 2011-07-13
    Changes: Version format compliance
  • Version 1.2: 2021-10-20
    Changes: Database references, Derived calculations
  • Version 1.3: 2024-02-21
    Changes: Data collection