Download MendeleyPromiscuity and specificity in DNA binding to SSB: Insights from the structure of the Mycobacterium smegmatis SSB-ssDNA complex.
Kaushal, P.S., Manjunath, G.P., Sekar, K., Muniyappa, K., Vijayan, M.To be published.
3A5U
Promiscuity and specificity in DNA binding to SSB: Insights from the structure of the Mycobacterium smegmatis SSB-ssDNA complex
- PDB DOI: https://doi.org/10.2210/pdb3A5U/pdb
- NAKB: 3A5U
- Classification: DNA BINDING PROTEIN
- Organism(s): Mycolicibacterium smegmatis MC2 155
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2009-08-12 Released: 2010-08-18
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.80 Å
- R-Value Free: 0.283
- R-Value Work: 0.238
- R-Value Observed: 0.240
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Single-stranded DNA-binding protein | 130 | Mycolicibacterium smegmatis MC2 155 | Mutation(s): 0 Gene Names: ssb |  | |
UniProt | |||||
Find proteins for Q9AFI5 (Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)) Explore Q9AFI5 Go to UniProtKB: Q9AFI5 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q9AFI5 | ||||
Sequence AnnotationsExpand | |||||
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Find similar nucleic acids by:
(by identity cutoff) | 3D Structure
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.80 Å
- R-Value Free: 0.283
- R-Value Work: 0.238
- R-Value Observed: 0.240
- Space Group: P 43 21 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 112.33 | α = 90 |
| b = 112.33 | β = 90 |
| c = 48.49 | γ = 90 |
| Software Name | Purpose |
|---|---|
| MAR345dtb | data collection |
| PHASER | phasing |
| REFMAC | refinement |
| MOSFLM | data reduction |
| SCALA | data scaling |
Entry History
Deposition Data
- Released Date: 2010-08-18 Deposition Author(s): Kaushal, P.S., Manjunath, G.P., Sekar, K., Muniyappa, K., Vijayan, M.
Revision History (Full details and data files)
- Version 1.0: 2010-08-18
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2023-11-01
Changes: Data collection, Database references, Refinement description
