3UPT
Crystal structure of a transketolase from Burkholderia pseudomallei bound to TPP, calcium and ribose-5-phosphate
- PDB DOI: https://doi.org/10.2210/pdb3UPT/pdb
- Classification: TRANSFERASE
- Organism(s): Burkholderia thailandensis E264
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2011-11-18 Released: 2011-12-14
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.40 Å
- R-Value Free: 0.200
- R-Value Work: 0.151
- R-Value Observed: 0.153
This is version 2.1 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Transketolase | 711 | Burkholderia thailandensis E264 | Mutation(s): 0 Gene Names: BTH_I1195, tkt EC: 2.2.1.1 |  | |
UniProt | |||||
Find proteins for Q2SZA7 (Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CCUG 48851 / CIP 106301 / E264)) Explore Q2SZA7 Go to UniProtKB: Q2SZA7 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q2SZA7 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 4 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| TPP Query on TPP | D [auth A], J [auth B] | THIAMINE DIPHOSPHATE C12 H19 N4 O7 P2 S AYEKOFBPNLCAJY-UHFFFAOYSA-O |  | ||
| RP5 Query on RP5 | E [auth A], F [auth A] | 5-O-phosphono-beta-D-ribofuranose C5 H11 O8 P KTVPXOYAKDPRHY-TXICZTDVSA-N |  | ||
| BR Query on BR | G [auth A], H [auth A], K [auth B], L [auth B] | BROMIDE ION Br CPELXLSAUQHCOX-UHFFFAOYSA-M |  | ||
| CA Query on CA | C [auth A], I [auth B] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.40 Å
- R-Value Free: 0.200
- R-Value Work: 0.151
- R-Value Observed: 0.153
- Space Group: P 41 21 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 146.98 | α = 90 |
| b = 146.98 | β = 90 |
| c = 142.87 | γ = 90 |
| Software Name | Purpose |
|---|---|
| XSCALE | data scaling |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| XDS | data reduction |
Entry History
Deposition Data
- Released Date: 2011-12-14 Deposition Author(s): Seattle Structural Genomics Center for Infectious Disease (SSGCID)
Revision History (Full details and data files)
- Version 1.0: 2011-12-14
Type: Initial release - Version 1.1: 2013-10-30
Changes: Database references - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Database references, Derived calculations - Version 2.1: 2023-09-13
Changes: Data collection, Database references, Refinement description, Structure summary
